Discription	Methacholine chloride is a synthetic choline ester that acts as a non-selective muscarinic receptor agonist in the parasympathetic nervous system.

Product Information

Catalog Num	A17285
Formula	C8H18ClNO2
Molecular Weight	195.69
CAS Number	62-51-1
SMILES	CC(C[N+](C)(C)C)OC(=O)C.[Cl-]
Synonyms	Methacholine Chloride; Acetyl-beta-methylcholine chloride; Provocholine; Mecholyl chloride; Amechol; Methapharma Brand of Methacholine Chloride; Provocholine; Provokit; Roche Brand of Methacholine Chloride;
Storage	Store lyophilized at -20ºC, keep desiccated. In lyophilized form, the chemical is stable for 36 months. In solution, store at -20ºC and use within 3 months to prevent loss of potency. Aliquot to avoid multiple freeze/thaw cycles.

Solubility

In vitro	DMSO	37 mg/mL (189.07 mM)
	Water	37 mg/mL (189.07 mM)
	Ethanol	37 mg/mL (189.07 mM)
* <1 mg/ml means slightly soluble or insoluble. * Please note that Adooq tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Preparing Stock Solutions

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
0.1 mM	51.1 mL	255.51 mL	511.01 mL
0.5 mM	10.22 mL	51.1 mL	102.2 mL
1 mM	5.11 mL	25.55 mL	51.1 mL
5 mM	1.02 mL	5.11 mL	10.22 mL

Useful Calculator

This calculator helps you calculate mass of compound based on solution concentration, volume and molecular weight in a specific solution using the formula:

Mass (mg) = Concentration (mM) × Volume (mL) × Molecular Weight (g/mol)

Calculate Reset

* Please check COA/MSDS for correct molecular weight.

Calculate the dilution required to prepare a stock solution.
This equation is commonly abbreviated as: C₁V₁ = C₂V₂