Product Data Sheet
Hoechst 33342 trihydrochloride
Catalog No. A24508
Hoechst 33342 trihydrochloride is a cell-permeable fluorescent dye from the Hoechst series, widely used as a live-cell nuclear marker. It binds preferentially to the minor groove of A/T-rich regions in double-stranded DNA, resulting in enhanced fluorescence intensity. Although it can associate with various nucleic acids, its fluorescence is significantly stronger upon binding to A/T-rich DNA. Hoechst 33342 is suitable for labeling live cells, and its fluorescence intensity increases with rising solution pH.
Chemical Information
| Catalog Num | A24508 |
|---|---|
| M. Wt | 561.93 |
| Formula | C27H31Cl3N6O |
| Purity | >98% |
| Storage | at -20 °C 3 years (powder form); at -20 °C 6 months (Solution base) |
| CAS No. | 875756-97-1 |
| Synonyms | |
| SMILES | [H]Cl.[H]Cl.[H]Cl.CN1CCN(C2=CC=C3C(N=C(C4=CC=C5C(N=C(C6=CC=C(OCC)C=C6)N5)=C4)N3)=C2)CC1 |
Biological Activity
| Description | Hoechst 33342 trihydrochloride is a cell-permeable fluorescent dye from the Hoechst series, widely used as a live-cell nuclear marker. It binds preferentially to the minor groove of A/T-rich regions in double-stranded DNA, resulting in enhanced fluorescence intensity. Although it can associate with various nucleic acids, its fluorescence is significantly stronger upon binding to A/T-rich DNA. Hoechst 33342 is suitable for labeling live cells, and its fluorescence intensity increases with rising solution pH. |
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RETURN POLICY
Adooq’s Unconditional Return Policy ensures a smooth online shopping experience for our customers. If you are in any way unsatisfied with your purchase, you may return any item(s) within 7 days of receiving it. In the event of product quality issues, either protocol related or product related problems, you may return any item(s) within 365 days from the original purchase date. Please follow the instructions below when returning products.
Adooq’s Unconditional Return Policy ensures a smooth online shopping experience for our customers. If you are in any way unsatisfied with your purchase, you may return any item(s) within 7 days of receiving it. In the event of product quality issues, either protocol related or product related problems, you may return any item(s) within 365 days from the original purchase date. Please follow the instructions below when returning products.
SHIPPING AND STORAGE
Adooq products are transported at room temperature. If you receive the product at room temperature, please rest assured, the Adooq Quality Inspection Department has conducted experiments to verify that the normal temperature placement of one month will not affect the biological activity of powder products. After collecting, please store the product according to the requirements described in the datasheet. Most Adooq products are stable under the recommended conditions.
Adooq products are transported at room temperature. If you receive the product at room temperature, please rest assured, the Adooq Quality Inspection Department has conducted experiments to verify that the normal temperature placement of one month will not affect the biological activity of powder products. After collecting, please store the product according to the requirements described in the datasheet. Most Adooq products are stable under the recommended conditions.
