Product Data Sheet
Fluorescein-5-maleimide
Catalog No. A24582
Fluorescein-5-maleimide (N-(5-Fluoresceinyl)maleimide) is a thiol-reactive fluorescent dye widely used for covalent labeling of proteins, peptides, and other biomolecules containing cysteine residues. By forming stable thioether bonds with sulfhydryl groups, it enables precise detection and quantification of thiol-containing targets.
Fluorescein-5-maleimide is employed to monitor the redox state of thiols in eukaryotic cells, label peptides or actin for protein–protein interaction studies—such as investigations of actin binding to cardiac myosin-binding protein C (cMyBP-C)—and to detect negatively charged nanoparticles. It also serves as a tool for screening mutant proteins containing cysteine residues. The dye exhibits strong fluorescence with excitation and emission maxima at 494 nm and 519 nm, respectively, making it highly suitable for fluorescence microscopy, flow cytometry, and biochemical assays.
Fluorescein-5-maleimide is employed to monitor the redox state of thiols in eukaryotic cells, label peptides or actin for protein–protein interaction studies—such as investigations of actin binding to cardiac myosin-binding protein C (cMyBP-C)—and to detect negatively charged nanoparticles. It also serves as a tool for screening mutant proteins containing cysteine residues. The dye exhibits strong fluorescence with excitation and emission maxima at 494 nm and 519 nm, respectively, making it highly suitable for fluorescence microscopy, flow cytometry, and biochemical assays.
Chemical Information
| Catalog Num | A24582 |
|---|---|
| M. Wt | 427.36 |
| Formula | C24H13NO7 |
| Purity | >98% |
| Storage | at -20 °C 3 years (powder form); at -20 °C 6 months (Solution base) |
| CAS No. | 75350-46-8 |
| Synonyms | N-(5-Fluoresceinyl)maleimide |
| SMILES | O=C(C=C1)N(C2=CC3=C(C4(C5=C(OC6=C4C=CC(O)=C6)C=C(O)C=C5)OC3=O)C=C2)C1=O |
Biological Activity
| Description | Fluorescein-5-maleimide (N-(5-Fluoresceinyl)maleimide) is a thiol-reactive fluorescent dye widely used for covalent labeling of proteins, peptides, and other biomolecules containing cysteine residues. By forming stable thioether bonds with sulfhydryl groups, it enables precise detection and quantification of thiol-containing targets. Fluorescein-5-maleimide is employed to monitor the redox state of thiols in eukaryotic cells, label peptides or actin for protein–protein interaction studies—such as investigations of actin binding to cardiac myosin-binding protein C (cMyBP-C)—and to detect negatively charged nanoparticles. It also serves as a tool for screening mutant proteins containing cysteine residues. The dye exhibits strong fluorescence with excitation and emission maxima at 494 nm and 519 nm, respectively, making it highly suitable for fluorescence microscopy, flow cytometry, and biochemical assays. |
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RETURN POLICY
Adooq’s Unconditional Return Policy ensures a smooth online shopping experience for our customers. If you are in any way unsatisfied with your purchase, you may return any item(s) within 7 days of receiving it. In the event of product quality issues, either protocol related or product related problems, you may return any item(s) within 365 days from the original purchase date. Please follow the instructions below when returning products.
Adooq’s Unconditional Return Policy ensures a smooth online shopping experience for our customers. If you are in any way unsatisfied with your purchase, you may return any item(s) within 7 days of receiving it. In the event of product quality issues, either protocol related or product related problems, you may return any item(s) within 365 days from the original purchase date. Please follow the instructions below when returning products.
SHIPPING AND STORAGE
Adooq products are transported at room temperature. If you receive the product at room temperature, please rest assured, the Adooq Quality Inspection Department has conducted experiments to verify that the normal temperature placement of one month will not affect the biological activity of powder products. After collecting, please store the product according to the requirements described in the datasheet. Most Adooq products are stable under the recommended conditions.
Adooq products are transported at room temperature. If you receive the product at room temperature, please rest assured, the Adooq Quality Inspection Department has conducted experiments to verify that the normal temperature placement of one month will not affect the biological activity of powder products. After collecting, please store the product according to the requirements described in the datasheet. Most Adooq products are stable under the recommended conditions.
