Product Data Sheet
DAPI
Catalog No. A24944
DAPI (4',6-Diamidino-2-phenylindole) is a fluorescent DNA dye that selectively binds to the AT base pairs in the minor groove of double-stranded DNA, enhancing fluorescence intensity approximately 20-fold upon binding. This property makes DAPI suitable for applications in fluorescence microscopy, allowing for quantification of DNA based on fluorescence intensity. Additionally, DAPI's ability to penetrate intact cell membranes enables its use in staining both live and fixed cells. DAPI also acts as an inhibitor of acid-sensing ion channel 3 (ASIC3), potentially contributing to research in chronic pain treatment (Ex/Em = 356/451 nm).
Chemical Information
| Catalog Num | A24944 |
|---|---|
| M. Wt | 277.32 |
| Formula | C16H15N5 |
| Purity | >98% |
| Storage | at -20 °C 3 years (powder form); at -20 °C 6 months (Solution base) |
| CAS No. | 47165-04-8 |
| Synonyms | 4',6-Diamidino-2-phenylindole |
| SMILES | N=C(C1=CC=C(C2=CC3=CC=C(C(N)=N)C=C3N2)C=C1)N |
Biological Activity
| Description | DAPI (4',6-Diamidino-2-phenylindole) is a fluorescent DNA dye that selectively binds to the AT base pairs in the minor groove of double-stranded DNA, enhancing fluorescence intensity approximately 20-fold upon binding. This property makes DAPI suitable for applications in fluorescence microscopy, allowing for quantification of DNA based on fluorescence intensity. Additionally, DAPI's ability to penetrate intact cell membranes enables its use in staining both live and fixed cells. DAPI also acts as an inhibitor of acid-sensing ion channel 3 (ASIC3), potentially contributing to research in chronic pain treatment (Ex/Em = 356/451 nm). |
|---|
RETURN POLICY
Adooq’s Unconditional Return Policy ensures a smooth online shopping experience for our customers. If you are in any way unsatisfied with your purchase, you may return any item(s) within 7 days of receiving it. In the event of product quality issues, either protocol related or product related problems, you may return any item(s) within 365 days from the original purchase date. Please follow the instructions below when returning products.
Adooq’s Unconditional Return Policy ensures a smooth online shopping experience for our customers. If you are in any way unsatisfied with your purchase, you may return any item(s) within 7 days of receiving it. In the event of product quality issues, either protocol related or product related problems, you may return any item(s) within 365 days from the original purchase date. Please follow the instructions below when returning products.
SHIPPING AND STORAGE
Adooq products are transported at room temperature. If you receive the product at room temperature, please rest assured, the Adooq Quality Inspection Department has conducted experiments to verify that the normal temperature placement of one month will not affect the biological activity of powder products. After collecting, please store the product according to the requirements described in the datasheet. Most Adooq products are stable under the recommended conditions.
Adooq products are transported at room temperature. If you receive the product at room temperature, please rest assured, the Adooq Quality Inspection Department has conducted experiments to verify that the normal temperature placement of one month will not affect the biological activity of powder products. After collecting, please store the product according to the requirements described in the datasheet. Most Adooq products are stable under the recommended conditions.
