Product Data Sheet
3'-O-(2-Nitrobenzyl)-dATP
Catalog No. A38660
3'-O-(2-Nitrobenzyl)-dATP is a photolabile DNA synthesis terminator that specifically targets the 3'-end of growing DNA strands. Its incorporation results in the cessation of DNA synthesis in a base-specific manner. Upon exposure to UV light, the 3'-protecting nitrobenzyl group is efficiently removed, allowing for the reinitiation of DNA synthesis. This compound is particularly valuable in the development of the Base Addition Sequencing Scheme (BASS), facilitating iterative stop-start cycles in DNA synthesis.
Chemical Information
| Catalog Num | A38660 |
|---|---|
| M. Wt | 626.30 |
| Formula | C17H21N6O14P3 |
| Purity | >98% |
| Storage | at -20 °C 3 years (powder form); at -20 °C 6 months (Solution base) |
| CAS No. | 159954-97-9 |
| Synonyms | |
| SMILES | NC1=NC=NC2=C1N=CN2[C@H]3C[C@H](OCC4=CC=CC=C4[N+]([O-])=O)[C@@H](COP(OP(OP(O)(O)=O)(O)=O)(O)=O)O3 |
Biological Activity
| Description | 3'-O-(2-Nitrobenzyl)-dATP is a photolabile DNA synthesis terminator that specifically targets the 3'-end of growing DNA strands. Its incorporation results in the cessation of DNA synthesis in a base-specific manner. Upon exposure to UV light, the 3'-protecting nitrobenzyl group is efficiently removed, allowing for the reinitiation of DNA synthesis. This compound is particularly valuable in the development of the Base Addition Sequencing Scheme (BASS), facilitating iterative stop-start cycles in DNA synthesis. |
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RETURN POLICY
Adooq’s Unconditional Return Policy ensures a smooth online shopping experience for our customers. If you are in any way unsatisfied with your purchase, you may return any item(s) within 7 days of receiving it. In the event of product quality issues, either protocol related or product related problems, you may return any item(s) within 365 days from the original purchase date. Please follow the instructions below when returning products.
Adooq’s Unconditional Return Policy ensures a smooth online shopping experience for our customers. If you are in any way unsatisfied with your purchase, you may return any item(s) within 7 days of receiving it. In the event of product quality issues, either protocol related or product related problems, you may return any item(s) within 365 days from the original purchase date. Please follow the instructions below when returning products.
SHIPPING AND STORAGE
Adooq products are transported at room temperature. If you receive the product at room temperature, please rest assured, the Adooq Quality Inspection Department has conducted experiments to verify that the normal temperature placement of one month will not affect the biological activity of powder products. After collecting, please store the product according to the requirements described in the datasheet. Most Adooq products are stable under the recommended conditions.
Adooq products are transported at room temperature. If you receive the product at room temperature, please rest assured, the Adooq Quality Inspection Department has conducted experiments to verify that the normal temperature placement of one month will not affect the biological activity of powder products. After collecting, please store the product according to the requirements described in the datasheet. Most Adooq products are stable under the recommended conditions.
