Product Data Sheet
2',7'-Difluorofluorescein
Catalog No. A43692
2',7'-Difluorofluorescein is a pH-sensitive fluorescent probe with a pKa of approximately 4.7. It provides ratiometric pH analysis through altered fluorescence intensity upon excitation at 488 nm, facilitating quantitative pH measurements between 2 and 7, particularly in submicron aerosol particles. This compound is notable for its ability to undergo buffer-mediated excited-state proton transfer and maintain stable fluorescence characteristics in physiological pH ranges, making it suitable for applications in fluorescent imaging, calcium indicator systems, and as a fluorescent protein label. Additionally, it demonstrates resistance to photodegradation, ensuring reliability in experimental settings.
Chemical Information
| Catalog Num | A43692 |
|---|---|
| M. Wt | 368.29 |
| Formula | C20H10F2O5 |
| Purity | >98% |
| Storage | at -20 °C 3 years (powder form); at -20 °C 6 months (Solution base) |
| CAS No. | 195136-58-4 |
| Synonyms | Oregon green 488 |
| SMILES | O=C1OC2(C3=C(OC4=C2C=C(F)C(O)=C4)C=C(O)C(F)=C3)C5=C1C=CC=C5 |
Biological Activity
| Description | 2',7'-Difluorofluorescein is a pH-sensitive fluorescent probe with a pKa of approximately 4.7. It provides ratiometric pH analysis through altered fluorescence intensity upon excitation at 488 nm, facilitating quantitative pH measurements between 2 and 7, particularly in submicron aerosol particles. This compound is notable for its ability to undergo buffer-mediated excited-state proton transfer and maintain stable fluorescence characteristics in physiological pH ranges, making it suitable for applications in fluorescent imaging, calcium indicator systems, and as a fluorescent protein label. Additionally, it demonstrates resistance to photodegradation, ensuring reliability in experimental settings. |
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RETURN POLICY
Adooq’s Unconditional Return Policy ensures a smooth online shopping experience for our customers. If you are in any way unsatisfied with your purchase, you may return any item(s) within 7 days of receiving it. In the event of product quality issues, either protocol related or product related problems, you may return any item(s) within 365 days from the original purchase date. Please follow the instructions below when returning products.
Adooq’s Unconditional Return Policy ensures a smooth online shopping experience for our customers. If you are in any way unsatisfied with your purchase, you may return any item(s) within 7 days of receiving it. In the event of product quality issues, either protocol related or product related problems, you may return any item(s) within 365 days from the original purchase date. Please follow the instructions below when returning products.
SHIPPING AND STORAGE
Adooq products are transported at room temperature. If you receive the product at room temperature, please rest assured, the Adooq Quality Inspection Department has conducted experiments to verify that the normal temperature placement of one month will not affect the biological activity of powder products. After collecting, please store the product according to the requirements described in the datasheet. Most Adooq products are stable under the recommended conditions.
Adooq products are transported at room temperature. If you receive the product at room temperature, please rest assured, the Adooq Quality Inspection Department has conducted experiments to verify that the normal temperature placement of one month will not affect the biological activity of powder products. After collecting, please store the product according to the requirements described in the datasheet. Most Adooq products are stable under the recommended conditions.
