Product Data Sheet

4-Nitrophenyl β-D-cellotetraoside

Catalog No. A44573

main product photo
4-Nitrophenyl β-D-cellotetraoside is a fluorescent dye that functions as a cellulose mimetic, comprising a tetramer of D-glucose units with β-1-4 glycosidic bonds. It serves as a substrate for enzymatic hydrolysis, allowing researchers to analyze the fragmentation pattern via thin-layer chromatography or by quantifying the release of 4-nitrophenol, which exhibits strong absorbance at 395 nm in alkaline solutions. This reagent is particularly useful in studies of cellulose degradation, enabling the assessment of cellulase specificity and activity.
Chemical Information
Catalog NumA44573
M. Wt787.67
FormulaC30H45NO23
Purity>98%
Storageat -20 °C 3 years (powder form); at -20 °C 6 months (Solution base)
CAS No.129411-62-7
Synonymsp-Nitopheyl β-D-cellotetaoside
SMILESOC[C@@H](O[C@H]1O[C@@H]([C@@H]([C@H]2O)O)[C@H](O[C@H]2OC(C=C3)=CC=C3[N](=O)=O)CO)[C@H]([C@@H]([C@H]1O)O)O[C@@H]([C@@H]([C@H]4O)O)O[C@@H]([C@H]4O[C@@H]([C@@H]([C@H]5O)O)O[C@@H]([C@H]5O)CO)CO
Biological Activity
Description4-Nitrophenyl β-D-cellotetraoside is a fluorescent dye that functions as a cellulose mimetic, comprising a tetramer of D-glucose units with β-1-4 glycosidic bonds. It serves as a substrate for enzymatic hydrolysis, allowing researchers to analyze the fragmentation pattern via thin-layer chromatography or by quantifying the release of 4-nitrophenol, which exhibits strong absorbance at 395 nm in alkaline solutions. This reagent is particularly useful in studies of cellulose degradation, enabling the assessment of cellulase specificity and activity.
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