When preparation of Z-VAD-FMK DMSO solution, Should I use standard grade or Sterile, Anhydrous DMSO for stock solutions?

It is highly recommended to use Cell Culture Grade, Anhydrous DMSO

• Why Anhydrous? The FMK (fluoromethyl ketone) group is susceptible to hydrolysis over time. Using anhydrous DMSO minimizes moisture-induced degradation, extending the shelf life of your stock.
• Why Sterile? Since Z-VAD-FMK is often used in long-term cell culture assays, starting with a sterile solvent prevents contamination risks. If you use non-sterile DMSO, you may need to filter the final working solution, which introduces potential loss of the compound.