PH797804 (2 μM) reduced attachment of mouse mammary tumor-derived cells to uncoated and collagen-coated surfaces after overnight pretreatment. [1]
In neonatal rat astrocytes aged in vitro, prolonged PH-797804 treatment (2 μM) was associated with lower GFAP, TNFα and phospho-p38 signals after oxygen-glucose deprivation. These marker results do not establish neuronal protection. [2]
Cellular suppression of p38-pathway phosphorylation
Cell-based study · Mouse mammary tumor-derived cancer cells [1]
PH-treated cells showed lower MK2 and Hsp27 phosphorylation than DMSO controls.
Literature-Reported Treatment Conditions
- Exposure duration
- 24 h
- Method
- Cell treatment followed by immunoblotting
- Vehicle
- DMSO control
- Reported limits
- Fig. 1A concentration is not specified in the inspected methods/caption; the 2 μM, 96 h SILAC exposure is a separate design.
Methods and source locations
Compound reported in the paper: PH-797804; Compound label in the paper: PH
Results concern the material reported by the authors; no Adooq lot, stereochemical composition, or performance was tested.
Fig. 1A and caption, article p. 5 (PDF p. 6); Results, identification of proteome/phosphoproteome changes; Immunoblotting methods, p. 3
MK2 and Hsp27 phosphorylation: Immunoblotting; DMSO control; p38α knockout comparator — Fig. 1A and caption, article p. 5 (PDF p. 6); Results, identification of proteome/phosphoproteome changes; Immunoblotting methods, p. 3
Attachment to uncoated and collagen-coated surfaces
Cell-based study · Mouse mammary tumor-derived cancer cells [1]
Attachment decreased on both surfaces compared with DMSO.
Literature-Reported Treatment Conditions
- Treatment concentration
- 2 μM
- Exposure duration
- Overnight pretreatment, then 1 h attachment assay
- Method
- Compound present during pretreatment and replating; compare uncoated and collagen-coated plates
- Vehicle
- DMSO
- Reported limits
- Exact overnight duration and DMSO percentage not specified in the selected method.
Methods and source locations
Compound reported in the paper: PH-797804; Compound label in the paper: PH
Results concern the material reported by the authors; no Adooq lot, stereochemical composition, or performance was tested.
Cell Attachment Assay, article p. 2; cell-adhesion Results, p. 10; Fig. 6A and caption, p. 12 (PDF p. 13)
Attached cells per field normalized to corresponding WT/DMSO condition: Crystal-violet assay; 1 h attachment; n = 4 biological replicates — Cell Attachment Assay, article p. 2; cell-adhesion Results, p. 10; Fig. 6A and caption, p. 12 (PDF p. 13)
Astrocyte markers after oxygen-glucose deprivation
Cell-based study · Primary neonatal Wistar rat astrocytes maintained to 30 DIV as an in vitro aging model [2]
PH-treated OGD cultures showed lower GFAP, TNFα and phospho-p38 bands than untreated OGD cultures.
Literature-Reported Treatment Conditions
- Treatment concentration
- 2 μM
- Exposure duration
- Added at seeding and each medium change; OGD performed at 30 DIV for 4 h
- Method
- Prolonged culture treatment followed by OGD and immunoblotting
- Reported limits
- Vehicle, independent replicate count for Fig. 4C, and compound maintenance during OGD/recovery medium exchange are not explicitly specified in the inspected text.
Methods and source locations
Compound reported in the paper: PH-797804; Compound label in the paper: PH
Results concern the material reported by the authors; no Adooq lot, stereochemical composition, or performance was tested.
Materials and Methods: Astrocyte primary cultures and Oxygen-glucose deprivation; treatment Results; Fig. 4C and caption
GFAP, TNFα and phospho-p38 protein signals after 4 h OGD: Immunoblotting; normoxic and OGD cultures with and without PH; α-actinin loading control — Materials and Methods: Astrocyte primary cultures and Oxygen-glucose deprivation; treatment Results; Fig. 4C and caption
