CEMT is a selective substrate for carboxylesterases, functioning as a ratiometric two-photon fluorescent reporter probe. Upon hydrolysis by carboxylesterases, CEMT generates HMT, which facilitates mitochondrial pH sensing through ratiometric fluorescence changes in response to pH fluctuations. This compound specifically targets and covalently binds to mitochondria, minimizing leakage during acidification, thereby enabling in situ imaging of mitochondrial dynamics and pH variations within biological systems.
CEMT is a selective substrate for carboxylesterases, functioning as a ratiometric two-photon fluorescent reporter probe. Upon hydrolysis by carboxylesterases, CEMT generates HMT, which facilitates mitochondrial pH sensing through ratiometric fluorescence changes in response to pH fluctuations. This compound specifically targets and covalently binds to mitochondria, minimizing leakage during acidification, thereby enabling in situ imaging of mitochondrial dynamics and pH variations within biological systems.
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