WH-15 is a fluorogenic phospholipase C (PLC) reporter that exhibits Km values of 49, 30, and 86.1 µM for PLC-γ1, PLC-δ1, and PLC-β2, respectively. This compound undergoes a cascade reaction to produce a fluorescent 6-aminoquinoline, enabling sensitive detection of PLC activity. WH-15 is primarily utilized for real-time imaging of PLC activity in live cells, with excitation and emission wavelengths of approximately 344 nm and 530 nm.
WH-15 is a fluorogenic phospholipase C (PLC) reporter that exhibits Km values of 49, 30, and 86.1 µM for PLC-γ1, PLC-δ1, and PLC-β2, respectively. This compound undergoes a cascade reaction to produce a fluorescent 6-aminoquinoline, enabling sensitive detection of PLC activity. WH-15 is primarily utilized for real-time imaging of PLC activity in live cells, with excitation and emission wavelengths of approximately 344 nm and 530 nm.
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