Dyes

BP Fluor 594 alkyne is a fluorescent dye designed for the specific imaging of azide-containing biomolecules. This probe utilizes a copper-catalyzed azide-alkyne cycloaddition (CuAAC) mechanism to react with azides, resulting in the formation of a stable triazole linkage. Its bright red fluorescence makes it an effective tool for various biological research applications, particularly in tracking biomolecular interactions and localization studies.

R6G phosphoramidite, 6-isomer, is a fluorescent dye that serves as a versatile building block for oligonucleotide synthesis. This xanthene dye from the rhodamine family exhibits a high fluorescence quantum yield and a significant molar extinction coefficient, making it ideal for a variety of labeling applications. With absorption and emission maxima shifted to longer wavelengths at 518 nm and 542 nm, respectively, R6G is particularly suitable for applications requiring enhanced fluorescence sensitivity and stability in nucleic acid studies and other biotechnological fields.

BODIPY 564/570 is a fluorescent dye that targets superparamagnetic nanoparticles (MNPs) for advanced imaging applications. With a maximal excitation wavelength of 564 nm and a maximal emission wavelength of 570 nm, this compound is highly suitable for various fluorescence-based studies. Its distinct optical properties make it ideal for cellular imaging and tracking of nanoparticle behavior in biological systems.

CDy9 is a selective fluorescent probe designed for the identification of mouse embryonic stem cells through fluorescence microscopy. This probe exhibits excitation and emission maxima at 563 nm and 578 nm, respectively, making it suitable for biological imaging applications. CDy9 plays a crucial role in stem cell research by enabling the visualization of cellular behaviors and characteristics in experimental settings.

C10 AV Ceramide (d18:1/10:0) is a fluorescent probe designed to measure CERT-mediated lipid transfer. The anthrylvinyl (AV) group functions as a fluorescent label attached to the amide-linked acyl chain of the ceramide molecule, facilitating visualization within cellular membranes. Its anthrylvinyl component localizes to the hydrophobic region of the lipid bilayer, enhancing the study of lipid dynamics and transport mechanisms in biological systems. This reagent is valuable for research in cell biology and membrane dynamics.

6-TAMRA cadaverine is a fluorescent dye primarily utilized for oligonucleotide labeling and automated DNA sequencing. The amino group (NH2) exhibits reactivity with carboxylic acids, activated NHS esters, and carbonyls, including ketones and aldehydes. This versatility makes 6-TAMRA cadaverine a valuable tool for various molecular biology applications, facilitating the visualization and characterization of nucleic acids.

R6G azide, 5-isomer is a xanthene-derived fluorescent dye characterized by a terminal azide group. This compound can undergo Click Chemistry with alkynes, BCN, and DBCO, forming a stable triazole linkage. Its unique structure and fluorescent properties make it suitable for a range of applications, including bioimaging and labelling studies in chemical research.

Pinacyanol chloride is a cyanine dye primarily utilized for its staining properties. It exhibits strong affinity for cellular components, making it effective in staining chromosomes in histological studies and bacteria in biochemical assays. This reagent is instrumental in enhancing the visualization of cell structures, aiding researchers in cellular and microbiological investigations.

Disperse Orange 3 is a monoazo fluorescent dye known for its notable absorption peak at 415 nm. This compound contains two aromatic rings, with one ring featuring an amino group and the other being modified with a nitro group. Disperse Orange 3 is primarily utilized in biological staining and imaging applications, making it suitable for research involving cell visualization and assay development.

Difluorocarboxyfluorescein NHS Ester, 6-isomer is an amine-reactive fluorescent dye known for its enhanced photostability and lower pKa (approximately 4.7), which renders its fluorescence largely pH insensitive within physiological ranges. This dye is particularly suitable for the labeling of proteins, peptides, and nucleotides, offering significant improvements in resolution during HPLC purification when utilizing a single isomer. Researchers benefit from the dye's favorable characteristics in various applications, enabling precise tracking and analysis of biomolecules in complex biochemical studies.

BP Fluor 532 Azide Plus is a fluorescent dye featuring a terminal azide group, primarily used in click chemistry applications. With a maximum absorption at 532 nm and emission at 554 nm, this compound exhibits robust fluorescence properties. Its unique structure incorporates a copper-chelating system, facilitating the formation of stable copper complexes that function as both reactants and catalysts in CuAAC (Copper-catalyzed Azide-Alkyne Cycloaddition) reactions, making it ideal for a variety of biological and chemical research applications.

Boc-Val-Arg-AMC is a synthetic peptide compound designed as a specific fluorescent substrate. It is selectively cleaved by enzymes such as thrombin, resulting in the release of the fluorescent molecule 7-amino-4-methylcoumarin (AMC). This compound is valuable for monitoring thrombin activity in various biological samples, including plasma, and has applications in enzyme activity assays and protease research.

Aniline blue diammonium is a vital dye commonly utilized in histological staining techniques, specifically for collagen fibers. It effectively stains collagen blue in tissue sections through Masson's trichrome protocol and is also employed for selective staining of callose in plant specimens. Additionally, Aniline blue diammonium is used in Gomori's one-step trichrome stain and Mallory's connective tissue stain, aiding in the assessment of nuclear maturity through histone staining in various tissues, including kidney and intestine.

3-(2-Iodoacetamido)-proxyl is a fluorescent dye that functions as a free radical spin probe, primarily utilized in spin labeling techniques. This compound is instrumental for investigating dynamic interactions within biological systems, including molecules, proteins, lipids, and cell membranes. Its properties make it valuable for research applications that require detailed analysis of molecular behavior in various environments.

BP Fluor 568 NHS ester is an amine-reactive fluorescent dye targeting primary amines in proteins, antibodies, and amine-modified oligonucleotides. With excitation and emission wavelengths of 578 nm and 602 nm, respectively, it enables robust labeling and tracking of biomolecules in various applications. This dye is ideal for use in fluorescence microscopy, flow cytometry, and other imaging techniques to study protein interactions and dynamics.

Perylene dU phosphoramidite is a photostable fluorescent dye derived from polycyclic aromatic hydrocarbons (PAHs) that exhibits a high quantum yield. Its fluorescence lifetime is short, preventing excimer formation and ensuring distinct signal generation. This reagent is designed for incorporation into DNA during automated oligonucleotide synthesis, enabling advanced imaging and detection applications in molecular biology and biochemistry research.

Filricianine sodium is a hydrophilic fluorochrome known for its ability to efficiently bind to antibodies at higher dye-to-protein (D/P) ratios. It exhibits excellent fluorescence properties, making it suitable for various research applications including western blotting, microscopy, flow cytometry, and cell-based assays. This reagent enables enhanced visualization and analysis of target molecules in biological samples.

Sulfo-Cy5.5-COOH is a sulfonated fluorescent dye characterized by the presence of both a sulfonic acid group and a carboxylic acid group. This dye exhibits strong fluorescence in the near-infrared region, making it suitable for applications requiring deep tissue penetration. It is commonly utilized in in vivo imaging, molecular labeling, and compatibility with various bioconjugation techniques.

TAMRA-PEG4-t-butyl ester is a fluorescent dye that targets red fluorescence, with an excitation and emission maxima of 553 nm and 575 nm, respectively. This compound features a t-butyl protected carboxyl group that is readily deprotected under acidic conditions. It is primarily utilized in cellular imaging and labeling applications, contributing to the study of biochemical processes and interactions within biological systems.

Erythrosine B aluminum is a fluorescent dye with colorimetric properties that targets a wide range of Gram-positive and Gram-negative bacteria. Its vibrant red color makes it suitable for live/dead cell assays, enabling the differentiation of viable from non-viable cells. This reagent is applicable in various experimental designs, including colorimetric and fluorescence-based assays, accommodating low to high-throughput screening needs.

C11-BODIPY 505/515 Glucosyl ceramide is a fluorescent lipid conjugate, labeled with C11-BODIPY 505/515, designed for advanced research applications in lipid nanoparticle formulation. This compound exhibits strong fluorescence properties with excitation/emission wavelengths of 505/515 nm, enabling tracking and visualization of lipid-based drug delivery systems. Its utility extends to studies on cellular uptake mechanisms and lipid metabolism, making it a valuable tool for researchers investigating lipid interactions in biological systems.

BP Fluor 647 Alkyne is a fluorescent dye characterized by its bright green fluorescence and optimal excitation with 633 and 650 nm Argon lasers. The alkyne moiety enables efficient reactions with azides through copper-catalyzed Click Chemistry. With its four sulfonate groups, this dye demonstrates excellent water solubility and reduced aggregation across a pH range of 4 to 10. BP Fluor 647 Alkyne is ideal for applications in protein and antibody labeling, as well as nucleic acid studies, allowing for high labeling density.

Tetra-sulfo-Cy7 DBCO is a highly fluorescent near-infrared dye designed for applications requiring low background noise and high sensitivity. This water-soluble, hydrophilic probe exhibits remarkable photostability and maintains consistent fluorescence across a pH range of 4 to 10, minimizing autofluorescence interference in biological samples. Tetra-sulfo-Cy7 DBCO is ideal for use in various imaging techniques, where its long-wavelength emission is optimized for detection by advanced imaging systems.

UBQ-3 NHS Ester is a fluorescent dye characterized by its terminal N-hydroxysuccinimide (NHS) ester group. The compound exhibits a broad emission spectrum ranging from 620 to 730 nm, making it an excellent acceptor for fluorescence resonance energy transfer (FRET) applications when paired with far-red to near-infrared emitting dyes, such as Cy5 and Alexa Fluor variants. This NHS ester can effectively label primary amines (-NH2) found on proteins, amine-modified oligonucleotides, and other amine-containing biomolecules, facilitating various biochemical and molecular biology research applications.

2-AS is a fluorescent probe designed to monitor the microenvironment and dynamic changes within biofilms. Its ability to provide real-time insights makes it essential for studying biofilm formation, stability, and behavior under varying conditions. This reagent is particularly valuable in microbiology and biotechnology research, facilitating a deeper understanding of microbial interactions and biofilm-related processes.

BP Fluor 488 Tetrazine is a bright green-fluorescent dye that serves as a highly effective probe for detecting TCO-tagged biopolymers. Its remarkable cycloaddition kinetics, reaching up to 30,000 M-1 s-1 with trans-cyclooctenes (TCO), make it the fastest reported for any bioorthogonal reaction. This compound is particularly valuable for applications requiring rapid reaction kinetics, such as in vivo cancer imaging and pre-targeted cell labeling studies.

BP Fluor 405 alkyne triTEA is a blue-fluorescent dye that serves as a versatile labeling reagent in multi-color applications, including flow cytometry and super-resolution microscopy techniques like STORM. It exhibits optimal excitation around the 407 nm spectral line of krypton lasers and the 408 nm violet laser diode. This dye participates in copper-catalyzed azide-alkyne cycloaddition (CuAAC), yielding a stable triazole linker upon reaction with azides. Additionally, a copper-free alternative, BP Fluor 405 DBCO, is available for applications sensitive to copper presence.

NBD-Hydrazine is a fluorescent probe that reacts specifically with aldehydes and ketones, exhibiting excitation and emission maxima at 468 nm and 535 nm, respectively. This compound is instrumental in the synthesis of malondialdehyde-reactive fluorescent probes, facilitating studies related to lipid peroxidation. Additionally, NBD-Hydrazine serves as a synthetic intermediate in the development of colorimetric sensors for cupric and chronic ions, enhancing research in analytical chemistry and biosensing applications.

ROX azide, 5-isomer is a red-emitting fluorescent dye known for its high brightness and fluorescence quantum yield. The presence of an azide group enables reactive conjugation with alkyne, BCN, and DBCO through Click Chemistry, resulting in a stable triazole linkage. This compound is widely utilized in cellular imaging and labeling applications, facilitating the study of biomolecular interactions and dynamics.

Carmine lithium is a natural red colorant derived from cochineal insects, classified within the coccid dye family. It is widely utilized in histological staining and as a biological marker due to its strong affinity for proteins and tissues. This reagent is essential for various research applications, including microscopy and cell imaging, enabling detailed visualization of biological samples.

BP Fluor 430 DBCO is a fluorescent dye that reacts with azides through a copper-free click chemistry mechanism, resulting in stable triazole formations without the need for copper catalysts or elevated temperatures. This dye exhibits bright, photostable green fluorescence, with optimal excitation at 432 nm and an emission peak at 539 nm, remaining pH independent across a broad pH range. BP Fluor 430 DBCO is suitable for various labeling applications in bioimaging and bioconjugation, although it is not recommended for staining intracellular components of fixed and permeabilized cells due to potential high background fluorescence.

BP Fluor 405 Picolyl Azide is a fluorescent dye designed for enhanced detection in bioconjugation reactions, utilizing a copper-chelating motif to increase local Cu(I) concentrations. This innovative reagent significantly accelerates the copper-catalyzed azide-alkyne cycloaddition (CuAAC) reaction rate while minimizing the required copper catalyst concentration by at least tenfold. The incorporation of the picolyl azide moiety allows for greater sensitivity in alkyne detection, making it particularly advantageous for applications involving low-abundance targets or those requiring amplified signal intensity. This makes BP Fluor 405 an essential tool for researchers focusing on advanced fluorescent labeling techniques.

Direct Brown 95 is a synthetic dye primarily utilized for coloring fabrics in the textile industry. Its applications extend to histology and cytology, where it aids in the differentiation of various cell types. Moreover, this dye serves as a molecular marker, enhancing the detection of nucleic acids such as DNA and RNA in laboratory analyses.

Solvent Red 52 is a fluorescent dye primarily utilized for its vibrant red color in various applications. It exhibits strong absorbance and fluorescence properties, making it suitable for use in the dyeing of plastic materials and other substrates. Additionally, this compound can be employed in biological research settings for tracking and visualizing cellular processes.

Toluidine Blue, an alkaline quinonimine dye, demonstrates a strong affinity for acidic tissue components, effectively staining nuclei blue and polysaccharides purple. This reagent exhibits heterostaining properties, enabling visualization of mast cells, mucins, and chondrocytes. Additionally, Toluidine Blue is employed in the histological analysis of plant tissues and is utilized as a diagnostic aid for identifying malignant lesions, including cancerous tissues. Its versatile staining capabilities make it a valuable tool in both medical and botanical research applications.

1,4-Bis(mesitylamino)anthraquinone is a multifunctional dye with notable applications in biological research. This compound serves as a vital tool for observing and analyzing cellular structures, tracking biomolecules, and evaluating cell functions. Its versatility extends to distinguishing cell types, detecting biomolecules, studying tissue pathology, and monitoring microorganisms. Beyond fundamental research, this dye is also employed in various clinical diagnostics and traditional textile dyeing, as well as in innovative areas such as functional textile processing, food pigmentation, and dye-sensitized solar cells.

Solvent Violet 9 is a DNA binding dye that intercalates between base pairs in double-stranded DNA. It exhibits fluorescence upon binding, making it useful for visualizing nucleic acids in various applications, including gel electrophoresis and fluorescence microscopy. This compound serves as an important tool for researchers studying DNA interactions, quantification, and overall nucleic acid analysis.

Relmapirazin is a fluorescent tracer agent primarily utilized for the real-time measurement of glomerular filtration rate (GFR). It facilitates the accurate assessment of kidney function in both preclinical and clinical research settings. Its ability to provide real-time tracking of renal filtration processes enhances the study of kidney diseases and nephrotoxicity in pharmacological research.

Karanjachromene is a fluorescent pyranoflavonoid derived from the seed oil of Pongamia pinnata. This compound exhibits significant biological activity as a photostable fluorescent probe. Its unique fluorescent properties make it valuable for various research applications, including cellular imaging and studying biological interactions in live cell systems.