Fluorescein-5-maleimide (N-(5-Fluoresceinyl)maleimide) is a thiol-reactive fluorescent dye widely used for covalent labeling of proteins, peptides, and other biomolecules containing cysteine residues. By forming stable thioether bonds with sulfhydryl groups, it enables precise detection and quantification of thiol-containing targets.
Fluorescein-5-maleimide is employed to monitor the redox state of thiols in eukaryotic cells, label peptides or actin for protein–protein interaction studies—such as investigations of actin binding to cardiac myosin-binding protein C (cMyBP-C)—and to detect negatively charged nanoparticles. It also serves as a tool for screening mutant proteins containing cysteine residues. The dye exhibits strong fluorescence with excitation and emission maxima at 494 nm and 519 nm, respectively, making it highly suitable for fluorescence microscopy, flow cytometry, and biochemical assays.
Fluorescein-5-maleimide (N-(5-Fluoresceinyl)maleimide) is a thiol-reactive fluorescent dye widely used for covalent labeling of proteins, peptides, and other biomolecules containing cysteine residues. By forming stable thioether bonds with sulfhydryl groups, it enables precise detection and quantification of thiol-containing targets.
Fluorescein-5-maleimide is employed to monitor the redox state of thiols in eukaryotic cells, label peptides or actin for protein–protein interaction studies—such as investigations of actin binding to cardiac myosin-binding protein C (cMyBP-C)—and to detect negatively charged nanoparticles. It also serves as a tool for screening mutant proteins containing cysteine residues. The dye exhibits strong fluorescence with excitation and emission maxima at 494 nm and 519 nm, respectively, making it highly suitable for fluorescence microscopy, flow cytometry, and biochemical assays.
Store lyophilized at -20ºC, keep desiccated. In lyophilized form, the chemical is stable for 36 months. In solution, store at -20ºC and use within 1 months to prevent loss of potency. Aliquot to avoid multiple freeze/thaw cycles.
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