Hoechst 33342 trihydrochloride is a cell-permeable fluorescent dye from the Hoechst series, widely used as a live-cell nuclear marker. It binds preferentially to the minor groove of A/T-rich regions in double-stranded DNA, resulting in enhanced fluorescence intensity. Although it can associate with various nucleic acids, its fluorescence is significantly stronger upon binding to A/T-rich DNA. Hoechst 33342 is suitable for labeling live cells, and its fluorescence intensity increases with rising solution pH.
Hoechst 33342 trihydrochloride is a cell-permeable fluorescent dye from the Hoechst series, widely used as a live-cell nuclear marker. It binds preferentially to the minor groove of A/T-rich regions in double-stranded DNA, resulting in enhanced fluorescence intensity. Although it can associate with various nucleic acids, its fluorescence is significantly stronger upon binding to A/T-rich DNA. Hoechst 33342 is suitable for labeling live cells, and its fluorescence intensity increases with rising solution pH.
Store lyophilized at -20ºC, keep desiccated. In lyophilized form, the chemical is stable for 36 months. In solution, store at -20ºC and use within 1 months to prevent loss of potency. Aliquot to avoid multiple freeze/thaw cycles.
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