Amyloid b-Peptide (1-42) (human)

Amyloid beta (Aβ or Abeta) is a peptide of 36-C43 amino acids that is processed from the Amyloid precursor protein. Amyloid β-Peptide (1-42) human is a 42-amino acid peptide which plays a key role in the pathogenesis of Alzheimer disease. Downregulates bcl-2 and increases the levels of bax. Neurotoxic.

Recommends the following protocol for re-suspending 100 µg of peptide into a 1 mg/mL solution:

• 1. Bring dried peptide to room temperature for 10 minutes.
• 2. Add 7.5 µL cold filter sterilized 1% Ammonium hydroxide (NH4OH) to the peptide aliquot, mix well by pipetting up and down. Take care to scrape inside lower walls of tube to ensure complete re-suspension of the film.
• 3. Add 92.5 µL of cold filter sterilized PBS. Optional - Centrifuge monomer 14,000 x g at 4 C for approximately 5-10 minutes and keep the supernatant to remove any material that was not fully re-suspended.
• 4. Keep monomer on ice and use immediately.
  
  * While it is not recommended, depending on your application small aliquots (≤ 50 μL) can be frozen and kept for up to 2 weeks at -80°C. However, aggregate should be removed by centrifugation at 4oC upon thawing.

Glenner and Wong (1984) Alzheimer's disease: initial report of the purification and characterization of a novel cerebrovascular amyloid protein. Biochem.Biophys.Res.Commun. 120 885 PMID: 6375662

Paradis et al (1996) Amyloid beta peptide of Alzheimer's disease downregulates bcl-2 and upregulates bax expression in human neurons. J.Neurosci. 16 7533 PMID: 8922409

Van Nostrand et al (1996) Amyloid β-protein induces the cerebrovascular cellular pathology of Alzheimer's disease and related disorders. Ann.N.Y.Acad.Sci. 777 297 PMID: 8624102