3'-O-(2-Nitrobenzyl)-dATP is a photolabile DNA synthesis terminator that specifically targets the 3'-end of growing DNA strands. Its incorporation results in the cessation of DNA synthesis in a base-specific manner. Upon exposure to UV light, the 3'-protecting nitrobenzyl group is efficiently removed, allowing for the reinitiation of DNA synthesis. This compound is particularly valuable in the development of the Base Addition Sequencing Scheme (BASS), facilitating iterative stop-start cycles in DNA synthesis.
3'-O-(2-Nitrobenzyl)-dATP is a photolabile DNA synthesis terminator that specifically targets the 3'-end of growing DNA strands. Its incorporation results in the cessation of DNA synthesis in a base-specific manner. Upon exposure to UV light, the 3'-protecting nitrobenzyl group is efficiently removed, allowing for the reinitiation of DNA synthesis. This compound is particularly valuable in the development of the Base Addition Sequencing Scheme (BASS), facilitating iterative stop-start cycles in DNA synthesis.
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