Cycloheximide (Actidione)

Catalog No.: A10036
Protein Synthesis Inhibitor
Cycloheximide (also known as actidione) is used as an selective antibiotic. It inhibits the protein synthesis (DNA-dependent RNA) of saprobic fungi eukaryotes. by binding with the 80S ribosome, while inactive against dermatophytes and systemic fungi.
Grouped product items
Size Price Stock Qty
200mg
$45.00
In stock
500mg
$65.00
In stock
Bulk Size
Bulk Discount
Free Delivery on orders over $500
Research use only. We do not sell to patients.
Prop65 Warning
WARNING

Attention California Residents: This product can expose you to chemicals including Cycloheximide (Actidione), which is known to the State of California to cause cancer and birth defects or other reproductive harm. For more information, go to www.P65Warnings.ca.gov

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Adooq Products cited in reputable paper
Science VOLUME 380, ISSUE 6663 (2023)
Science VOLUME 369, ISSUE 6510 (2020)
Science VOLUME 356, ISSUE 6336 (2017)
Cell Vol. 185 Issue 23 p4428-4447.e28
Cell Vol 177, Issue 7, p1933-1947.e25
Cell Vol 156, Issue 5, p857-1114
Nature Volume 622 Issue 7982 (2023)
Nature volume 620, pages890-897 (2023)
Nature volume 610, pages540-546 (2022)
Nature volume 588, pages83-88 (2020)
Nature volume 574, pages268-272 (2019)
Nature volume 573, pages539-545 (2019)
Nature volume 567, pages118-122 (2019)
Nature volume 551, pages639-643 (2017)
Nature volume 551, pages247-250 (2017)
Nature volume 548, pages356-360 (2017)
Nature volume 545, pages187-192 (2017)
Adooq's Cycloheximide (Actidione) has been cited by 12 publications
  • Xiao-Yi Zhang, .et al. , Free Radic Biol Med, 2025, Aug 25:S0891-5849(25)00940-2 PMID: 40865778
  • Wan-Cheng Liu, .et al. , Nat Commun, 2025, Jun 3;16(1):5154 PMID: 40461504
  • Xingmei Shu, .et al. , Chin Med J (Engl), 2025, May 23 PMID: 40405358
  • Jing Wang, .et al. , Mol Cell, 2025, May 15;85(10):1999-2016.e11 PMID: 40328247
  • Yang Liao, .et al. , Cell Signal, 2025, Jul:131:111735 PMID: 40081551
  • Xiangdong Qiu, .et al. , Cardiovascular Toxicology, 2025, 25: 427-440
  • Tianfeng Huang, .et al. , Mol Neurobiol, 2024, Sep 13 PMID: 39271624
  • Han Xiao, .et al. , JPA, 2023, Apr 19
  • Shuanghui Lu, .et al. , Chem Biol Interact, 2022, Dec 1;368:110219 PMID: 36243147
  • Ke Chen, .et al. , Cancer Commun (Lond), 2021, Apr; 41(4): 316-332 PMID: 33591636
  • Zhiyuan Qin, .et al. , Cancers (Basel), 2021, Feb 4;13(4):618 PMID: 33557163
  • Hua Xu, .et al. , Cancers (Basel), 2020, Apr; 12(4): 831 PMID: 32235588
Biological Activity
DescriptionCycloheximide (also known as actidione) is used as an selective antibiotic. It inhibits the protein synthesis (DNA-dependent RNA) of saprobic fungi eukaryotes. by binding with the 80S ribosome, while inactive against dermatophytes and systemic fungi.
Product Information
Catalog NumA10036
FormulaC15H23NO4
Molecular Weight281.4
CAS Number66-81-9
SMILESC[C@H]1C[C@@H](C(=O)[C@@H](C1)[C@@H](CC2CC(=O)NC(=O)C2)O)C
Storage

Store lyophilized at -20ºC, keep desiccated.
In lyophilized form, the chemical is stable for 36 months.
In solution, store at -20ºC and use within 1 months to prevent loss of potency. Aliquot to avoid multiple freeze/thaw cycles.

Solubility
In vitro DMSO48 mg/mL (170.61 mM)
Water13 mg/mL (46.2 mM)
Ethanol48 mg/mL
* <1 mg/ml means slightly soluble or insoluble.
* Please note that Adooq tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.
Preparing Stock Solutions
Concentration / Solvent Volume / Mass1 mg5 mg10 mg
0.1 mM35.54 mL177.68 mL355.37 mL
0.5 mM7.11 mL35.54 mL71.07 mL
1 mM3.55 mL17.77 mL35.54 mL
5 mM0.71 mL3.55 mL7.11 mL
Useful Calculator

This calculator helps you calculate mass of compound based on solution concentration, volume and molecular weight in a specific solution using the formula:

Mass (mg) = Concentration (mM) × Volume (mL) × Molecular Weight (g/mol)

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Please check COA/MSDS for correct molecular weight.

Calculate the dilution required to prepare a stock solution.
This equation is commonly abbreviated as: C1V1 = C2V2

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