Guanosine rescue of MMF-associated human Tenon fibroblast growth inhibition
Cell-based study · Primary human Tenon fibroblasts from cataract-surgery tissue explants, passages 3–7 [1]
MMF reduced cell accumulation in a concentration-dependent manner. At day 14, cell counts were 71% (SD 6.4) and 7.7% (SD 0.9) of control at 1 and 10 μM, respectively (both p<0.001). Supplementation with 100 μM guanosine restored counts to values statistically equal to control. Four separate experiments were performed in triplicate; these are not 12 independent donors.
| Endpoint | Reported result | Reference |
|---|---|---|
| Day-14 cell count at 1 μM MMF, relative to the same-day untreated control | Relative cell count 71% (SD 6.4); p<0.001 versus control (100%) | [1] |
| Day-14 cell count at 10 μM MMF, relative to the same-day untreated control | Relative cell count 7.7% (SD 0.9); p<0.001 versus control (100%) | [1] |
| MMF-treated cell counts with 100 μM guanosine | Guanosine rescue Statistically equal to control; no exact rescue percentage or P value reported in text | [1] |
Literature-Reported Treatment Conditions
- Treatment concentration
- MMF: 1 or 10 μM; guanosine rescue: 100 μM
- Exposure duration
- Cell counts at 7, 14 and 21 days; exact numerical extraction restricted to day 14
- Method
- Incubation in culture medium; manual counts of bisbenzimide-stained cells in six visual fields per well
- Co-treatment
- Guanosine was a mechanistic rescue condition, with untreated and guanosine-only controls.
- Reported limits
- Separate solvent/vehicle control and donor count are not specified. Salt form of the IV preparation is not stated. No MPA concentration, MMF hydrolysis, intracellular guanosine pool or direct IMPDH activity was measured.
Methods and source locations
Compound reported in the paper: Mycophenolate mofetil; Compound label in the paper: MMF; CellCept solution for intravenous application (Roche)
Evidence for the reported formulated MMF in cultured fibroblasts; no surgical efficacy or Adooq lot claim.
Materials and Method: Cell proliferation studies with guanosine; Results (day 14); Figure 2 and caption
Day-14 cell count at 1 μM MMF, relative to the same-day untreated control: Manual fluorescence cell counting; four separate experiments in triplicate; Figure 2 data expressed as means (SD). — Materials and Method: Cell proliferation studies with guanosine; Results (day 14); Figure 2 and caption
Day-14 cell count at 10 μM MMF, relative to the same-day untreated control: Manual fluorescence cell counting; four separate experiments in triplicate; Figure 2 data expressed as means (SD). — Materials and Method: Cell proliferation studies with guanosine; Results (day 14); Figure 2 and caption
MMF-treated cell counts with 100 μM guanosine: Manual fluorescence cell counting; four separate experiments in triplicate; Figure 2 data expressed as means (SD). — Materials and Method: Cell proliferation studies with guanosine; Results (day 14); Figure 2 and caption