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Fig. 1 · Structure
Cat. No.
A10751
CAS No.
50-24-8
Formula
C21H28O5
Mol. Weight
360.44

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Prednisolone

Cat. No. A10751 CAS 50-24-8
Prednisolone is a glucocorticoid with the general properties of the corticosteroids.

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Biological Activity

Cellular Effect

Cell Line Activity Value Description Reference
A549 EC50 0.002 nM Agonist activity at human mineralocorticoid receptor expressed in human A549 cells by fluorescence polarization assay [PMID: 21899328]
A549 EC50 13.6 nM Transrepression activity of glucocorticoid receptor in human A549 cells assessed as inhibition of IL1beta-stimulated NFkappaB-dependent E-selectin transcription by luciferase reporter gene assay [PMID: 21073190]
A549 EC50 13.6 nM Transrepression activity at GR in IL-1-beta-stimulated human A549 cells assessed as inhibition of NF-kappaB-dependent E-selectin transcription by luciferase reporter gene assay [PMID: 19321341]
A549 EC50 14.4 nM Transrepression activity at glucocorticoid receptor alpha in IL-1beta-stimulated human A549 cells assessed as inhibition of NFkappaB-dependent E-selectin transcription by luciferase reporter gene assay [PMID: 21899328]
A549 EC50 15.8 nM Transrepression activity of glucocorticoid receptor in human A549 cells expressing AP-1 assessed as inhibition of PMA-induced AP-1 activity by luciferase reporter gene assay [PMID: 21073190]
A549 EC50 16.1 nM Transrepression activity at glucocorticoid receptor alpha in phorbol myristate acetate-stimulated human A549 cells assessed as inhibition of AP1 response element by luciferase reporter gene assay [PMID: 21899328]
A549 IC50 5.8 nM Antiinflammatory activity in human A549 cells assessed as inhibition of IL1beta-induced IL6 production preincubated for 30 mins followed by IL1beta-stimulation and measured after 20 hrs by AlphaLisa assay [PMID: 30384041]
C2C12 EC50 3.4 nM Inhibition of mouse glutamine synthetase by GR-mediated transactivation in C2C12 cells [PMID: 15808489]
CV-1 ED50 4 nM Activity at human glucocorticoid receptor expressed in CV1 cells assessed as repression of TNF and IL-1-beta-induced E-selectin gene expression by luciferase reporter gene assay [PMID: 18032610]
HeLa EC50 16 nM Activation of MMTV in HeLa cells measured by luciferase activity [PMID: 17181172]
HeLa EC50 21 nM Transactivation activity at GR-alpha in human NP1 Hela cells assessed as inhibition of GAL4-DBD after 20 hrs by luciferase reporter gene assay [PMID: 21899328]
HeLa EC50 73.9 nM Transactivation activity at GR-alpha in human NP1 Hela cells assessed as induction of GAL4-DBD after 20 hrs by luciferase reporter gene assay [PMID: 21899328]
HeLa EC50 82.7 nM Transactivation activity of glucocorticoid receptor ligand binding domain expressed in human NP-1 Hela cells co-expressing GAL4 DNA binding domain by luciferase reporter gene assay [PMID: 21073190]
HepG2 IC50 23 nM Transrepression activity at glucocorticoid receptor in IL-1beta-stimulated human HepG2 cells assessed as inhibition of AP1 response element-induced IL-6 production by ELISA [PMID: 21115247]
HepG2 EC50 26 nM Agonist activity at glucocorticoid receptor in human HepG2 cells co-transfected with PEPCK assessed as GRE activation by luciferase reporter gene assay [PMID: 21324689]
HepG2 IC50 4.1 nM Transrepression activity at GR expressed in IL-1beta- and TNFalpha-stimulated HepG2 cells assessed as inhibition of NFKB- or AP-1 mediated E-selectin transcription by luciferase reporter gene assay [PMID: 21349714]
HepG2 IC50 4.1 nM Transrepression activity at glucocorticoid receptor in human HepG2 cells assessed as inhibition of TNFalpha/IL1beta-stimulated NFkappaB-dependent E-selectin repression by luciferase reporter gene assay [PMID: 21324689]
HepG2 IC50 4.1 nM Agonist activity at GR expressed in IL-1beta- and TNFalpha-stimulated HepG2 cells assessed as inhibition of NFKB- or AP-1 mediated E-selectin transcription by luciferase reporter gene assay relative to Dexamethasone [PMID: 21316964]

18 records · Scroll to view more

In Vitro Studies

Prednisolone increased IL-10 production in cultured CCR6+MDR1−/Rh123hi or CCR6−MDR1−/Rh123hi cells compared with DMSO. In cultured FACS-sorted CCR6+MDR1+/Rh123lo cells, Prednisolone did not increase IL-10 production compared with DMSO. Both studies assessed IL-10 expression by intracellular staining and flow cytometry. [1]

Literature-reported observations and conditions; not Adooq-tested protocols or general treatment recommendations.

Prednisolone increased IL-10 production in MDR1− memory T cells

Cell-based study · CCR6+MDR1−/Rh123hi, or CCR6−MDR1−/Rh123hi cells [1]

In cultured CCR6+MDR1−/Rh123hi or CCR6−MDR1−/Rh123hi cells, Prednisolone increased IL-10 production compared with DMSO. IL-10 expression was assessed by intracellular staining and flow cytometry.

Measured endpoints — not treatment concentrations
EndpointReported resultReference
IL-10 expression was determined by intracellular staining and flow cytometryIL-10 production CCR6+MDR1−/Rh123hi or CCR6−MDR1−/Rh123hi; prednisolone (Pred; 1 µM), cultured for 5 d: increased IL-10 production between two- and fourfold[1]

Literature-Reported Treatment Conditions

Treatment concentration
1 µM
Exposure duration
5 d
Method
cultured
Vehicle
vehicle control; DMSO
Co-treatment
Other interventions in this design: anti-CD3/anti-CD28; PMA 10 nM; ionomycin 1 µM. Compound effect isolated by the comparison: DMSO.
Methods and source locations

Compound reported in the paper: Prednisolone

Literature evidence for the named compound in the stated model; no Adooq product-use or lot-performance claim.

RESULTS > MDR1+ Th17.1 cells are resistant to glucocorticoid-mediated T cell suppression (S113); MATERIALS AND METHODS > Untitled > Media and cell culture. (S128); MATERIALS AND METHODS > Untitled > FACS sorting. (S147); MATERIALS AND METHODS > Untitled > FACS analysis. (S154); MATERIALS AND METHODS > Untitled > MDR1 activity assay. (S156); Figure 5. legend (S296, S297)

IL-10 expression was determined by intracellular staining and flow cytometry: IL-10 expression was determined by intracellular staining and flow cytometry; comparator: DMSO — RESULTS > MDR1+ Th17.1 cells are resistant to glucocorticoid-mediated T cell suppression (S113); Figure 5. legend (S296, S297)

Prednisolone does not increase IL-10 production in MDR1+ T cells

Cell-based study · FACS-sorted CCR6+MDR1+/Rh123lo [1]

In cultured FACS-sorted CCR6+MDR1+/Rh123lo cells, Prednisolone did not increase IL-10 production compared with DMSO. IL-10 expression was assessed by intracellular staining and flow cytometry.

Measured endpoints — not treatment concentrations
EndpointReported resultReference
IL-10 expression was determined by intracellular staining and flow cytometryReported result Prednisolone (Pred; 1 µM), cultured for 5 d: prednisolone increased IL-10 production by neither MDR1+ T cells[1]

Literature-Reported Treatment Conditions

Treatment concentration
1 µM
Exposure duration
5 d
Method
cultured
Vehicle
vehicle control; DMSO
Co-treatment
Other interventions in this design: anti-CD3/anti-CD28; PMA 10 nM; ionomycin 1 µM. Compound effect isolated by the comparison: DMSO.
Methods and source locations

Compound reported in the paper: Prednisolone

Literature evidence for the named compound in the stated model; no Adooq product-use or lot-performance claim.

RESULTS > MDR1+ Th17.1 cells are resistant to glucocorticoid-mediated T cell suppression (S114); MATERIALS AND METHODS > Untitled > Media and cell culture. (S128); MATERIALS AND METHODS > Untitled > FACS sorting. (S147); MATERIALS AND METHODS > Untitled > FACS analysis. (S154); MATERIALS AND METHODS > Untitled > MDR1 activity assay. (S156); Figure 5. legend (S296, S297)

IL-10 expression was determined by intracellular staining and flow cytometry: IL-10 expression was determined by intracellular staining and flow cytometry; comparator: DMSO — RESULTS > MDR1+ Th17.1 cells are resistant to glucocorticoid-mediated T cell suppression (S114); Figure 5. legend (S296, S297)

Specifications

Manufacturer Adooq Bioscience
CAS Number 50-24-8
Formula C21H28O5
Molecular Weight 360.44
SMILES
C[C@@]1([C@@]2(O)C(CO)=O)[C@](CC2)([H])[C@@](CCC3=CC4=O)([H])[C@]([C@]3(C=C4)C)([H])[C@@H](O)C1

Storage & Solubility

In Vitro Solubility

Reported Solubility

SolventReported Solubility
DMSO 66 mg/mL (183.1 mM)
Water Insoluble
Ethanol 9 mg/mL

Preparing Stock Solutions

Stock preparation is unavailable for this product molecular weight.

Calculated stock concentrations do not establish solvent solubility. Refer to the reported solubility data for experimental guidance.

Storage

Store powder at -20°C, keep desiccated.
In powder form, the chemical is stable for 36 months.
In solution, store at -20°C and use within 1 month to prevent loss of potency. Aliquot to avoid multiple freeze/thaw cycles.

Research References

  1. [1] Ramesh R, Kozhaya L, McKevitt K, et al. Pro-inflammatory human Th17 cells selectively express P-glycoprotein and are refractory to glucocorticoids. J Exp Med (2014). PMID: 24395888 DOI: 10.1084/jem.20130301 Source material

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