Catalog No.
Product Name
Application
Product Information
Citations
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Fluorescent Membrane-staining Dye
Di-4-ANEPPDHQ is a polarity-sensitive membrane probe designed for visualizing membrane microdomains in living cells. This doubly charged fluorescent dye enables the assessment of lipid accumulation by producing a fluorescence spectrum shift in response to changes in the polarity of the lipid environment upon embedding within the cell membrane. With an excitation wavelength of 488 nm and emission wavelengths of 560 nm and 650 nm corresponding to liquid ordered and disordered phases, Di-4-ANEPPDHQ is ideal for fluorescence imaging studies focusing on membrane microstructure, lipid order, and membrane tension response. -
Amyloid-β Inhibitor
Hoechst 34580 tetrahydrochloride is a nuclear marker dye that selectively targets A/T-rich double-stranded DNA. This compound exhibits enhanced fluorescence intensity when bound to nucleic acids, making it valuable for live cell labeling applications. As the pH of the solution increases, the fluorescence intensity of Hoechst 34580 also increases, providing a reliable tool for studying cellular dynamics, DNA distribution, and nuclear morphology in real-time. This reagent is particularly useful in research focused on amyloid-β and related neurodegenerative processes. -
Fluorescent Probe
DAF-2DA (5,6-Diaminofluorescein diacetate) is a fluorescent probe designed for the selective detection of nitric oxide (NO) in biological samples. Upon reaction with NO, DAF-2DA is converted into a highly fluorescent compound, allowing for quantitative measurement of NO levels. This reagent is widely utilized in cellular and physiological studies to investigate NO signaling pathways and its role in various biological processes. -
H2O2 probe
Peroxy Orange 1 is a hydrogen peroxide (H2O2) specific probe designed for the detection of reactive oxygen species. It binds selectively to highly reactive oxygen species, enabling the visualization of H2O2 in live cells through green fluorescence. This compound is valuable for studying oxidative stress, cellular signaling, and related biological processes in various research applications. -
Cell Membrane Staining Agent
Octadecyl Rhodamine B chloride is a versatile amphiphilic fluorescent probe targeting cell membranes. This compound effectively stains cell membranes and is instrumental in membrane biology research. It exhibits fluorescence properties, with a maximum absorption and emission wavelength of 565 nm and 585 nm, respectively, when in Triton X-100. In aqueous solutions, the compound forms aggregates that shift absorption peaks to 524 nm and 574 nm, resulting in notable fluorescence quenching. -
Mitochondria Fluorescent Dye
MitoTracker Orange CMTMRos is a fluorescent dye that selectively labels mitochondria in live cells by exploiting the mitochondrial membrane potential (Ex/Em: 551/576 nm). This compound is essential for studying mitochondrial dynamics, morphology, and function. It is widely used in research applications, including cellular metabolism, apoptosis, and drug-response assays in various cell types. -
Zinc Ion Fluorophore
Zinquin ethyl ester is a zinc ion fluorophore specifically designed for the detection of intracellular zinc ions. This lipophilic probe readily penetrates cell membranes and exhibits a strong affinity for Zn2+, resulting in blue fluorescence upon binding. Primarily utilized in research applications focused on zinc signaling and cellular metal ion dynamics, Zinquin ethyl ester serves as a valuable tool for studying zinc-related biological processes. -
Lipophilic Fluorochrome
CellTracker CM-DiI is a lipophilic fluorochrome designed for the visualization and tracking of lymphocyte migration. This reagent enables the labeling of cells for detection in histological sections, with an excitation wavelength of 553 nm and an emission wavelength of 570 nm. Its application is critical in studies examining cellular dynamics and immune responses. -
Fluorogenic Ligand
DFHO is a fluorogenic ligand specifically designed for binding with the Corn fluorogenic aptamer. With a Kd value of 70 nM, DFHO undergoes a conformational change upon binding, resulting in enhanced fluorescence, thereby facilitating RNA imaging within cellular environments. This compound is valuable for researchers investigating RNA dynamics and localization in live cell assays. -
Mitochondrial ROS Indicator
MitoSOX Red is a live cell fluorescent probe that specifically targets mitochondria and is cell membrane permeable. MitoSOX Red enters mitochondria and is oxidized by superoxide but not by other ROS or RNS generating systems. The oxidized MitoSOX Red then binds to nucleic acids in mitochondria/nucleus, producing strong red fluorescence. MitoSOX Red can be used as a fluorescent indicator to specifically detect superoxide. In addition, superoxide dismutase (SOD) can prevent the oxidation of MitoSOX Red. Excitation/emission wavelength: 510/580 nm. -
Hydrophobic Fluorescent Stain
Nile red (Nile blue oxazone) is a lipophilic stain. Nile red has environment-sensitive fluorescence. Nile red is intensely fluorescent in a lipid-rich environment while it has minimal fluorescence in aqueous media. Nile red is an excellent vital stain for the detection of intracellular lipid droplets by fluorescence microscopy and flow cytof uorometry. Nile red stains intracellular lipid droplets red. The fluorescence wavelength is 559/635 nm. -
Fluorochrome
Propidium Iodide (PI) is a nuclear staining agent that stains DNA. Propidium Iodide is an analogue of ethidine bromide that emits red fluorescence upon embedding in double-stranded DNA. Propidium Iodide cannot pass through living cell membranes, but it can pass through damaged cell membranes to stain the nucleus. Propidium Iodide has a fluorescence wavelength of 493/617 nm and a wavelength of 536/635 nm after Mosaic with DNA. Propidium Iodide is commonly used in the detection of apoptosis (apoptosis) or necrosis (necrosis), and is often used in flow cytometry analysis. -
Green Dye
BODIPY493/503 methyl bromide is a BODIPY dye. BODIPY dye is a small molecule dye with strong ultraviolet absorption ability, its fluorescence peak is relatively sharp, and the quantum yield is high. They are relatively insensitive to the polarity and pH of the environment and are relatively stable under different physiological conditions. Due to its structural asymmetry, BODIPY derives a variety of structural products. BODIPY lipid droplet dyes can well pass through the cell membrane into the cell, and localize the neutral lipids in the cell to specifically stain the lipid droplets, which can be used for labeling of live cells and fixed cells. Maximum excitation/emission wavelength: 493/503 nm. -
Neutral Lipid Dye
BODIPY 493/503 is a member of the BODIPY (boron-dipyrromethene) family of small-molecule fluorescent dyes, characterized by strong absorption in the ultraviolet-visible range, narrow and well-defined emission spectra, and high fluorescence quantum yields. These dyes exhibit minimal sensitivity to environmental polarity and pH, maintaining stable photophysical properties under a wide range of physiological conditions.
Due to the structural versatility of the BODIPY core, numerous derivatives with tailored properties can be generated. BODIPY-based lipid droplet probes readily permeate cellular membranes and selectively localize to neutral lipid compartments, enabling specific staining of intracellular lipid droplets. This makes them suitable for both live-cell imaging and fixed-cell applications.
Maximum excitation/emission wavelengths: 493/503 nm.
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Voltage-Sensitive Dye
Di-4-ANEPPS is a voltage-sensitive dye that targets voltage-gated ion channels, primarily inhibiting sodium currents. This compound alters fluorescence properties in response to changes in membrane potential, facilitating the examination of ion channel function. It is useful in cardiovascular research, particularly in studies involving electrophysiology of cardiomyocytes, myocardial ischemia, and investigating drug effects on cardiac cells. Di-4-ANEPPS is instrumental in assessing drug cardiotoxicity and elucidating the mechanisms underlying arrhythmias. -
Anti-microbial Agent/Kir3.2 Blocker
3,6-Diaminoacridine hemisulfate is an acridine compound that functions primarily as a broad-spectrum antimicrobial agent and a Kir3.2 potassium channel blocker. Its mechanism involves insertion into bacterial DNA, thereby disrupting replication and transcription, which ultimately leads to bacterial lysis. Additionally, this compound is utilized in research to investigate the neurological phenotype associated with Down syndrome. Due to its ability to penetrate the stratum corneum and accumulate in the cell nucleus, prolonged exposure warrants caution due to potential oncogenic effects. -
Fluorescent Tracer
Fluorescein is a fluorescent tracer commonly employed in medicinal and biological research, particularly for visualizing tumor-infected tissues. As a representative green fluorophore, it serves as a versatile scaffold for the development of various green fluorescent probes. Its high solubility and brightness make it an excellent choice for applications in microscopy and flow cytometry, facilitating studies in cellular and molecular biology. -
ROS Probe
HKOH-1 is a sensitive green fluorescent probe designed for the selective detection of hydroxyl radicals (·OH) in living cells, utilizing a maximum excitation wavelength of 500 nm and an emission wavelength of 520 nm. This reagent enables researchers to monitor oxidative stress and assess cellular responses to reactive oxygen species. Its application is critical in studies related to redox biology, neurodegenerative diseases, and cancer research. -
mtTrxR2 Probe
Mito-TRFS is an innovative off-on probe designed to selectively image mitochondrial thioredoxin reductase (TrxR2) in live cells. This reagent allows for the assessment of the redox status within mitochondria, facilitating the study of oxidative stress and mitochondrial function. Mito-TRFS is particularly valuable in research focusing on cellular responses to stress and the roles of TrxR2 in various disease models. -
Fluorescent Probe
DND-189 is a low-pH fluorescent probe that exhibits high sensitivity to neutral and acidic pH environments. This reagent is valuable for the quantification of pH levels within acidic organelles, enabling researchers to study intracellular pH dynamics in various biological contexts. Its unique properties make DND-189 an essential tool for applications in cellular imaging and pH monitoring. -
NO Fluorescent Detector
4,5-Diaminofluorescein is a sensitive fluorescent detector that specifically targets nitric oxide (NO). This reagent exhibits robust fluorescence upon reaction with NO, enabling the visualization and measurement of NO levels in cells and tissues. It is widely used in research applications related to nitric oxide signaling, endothelial function, and various physiological processes. -
Fluorescent Dye
ER Tracker Yellow is a temperature-sensitive fluorescent dye specifically designed to target the endoplasmic reticulum (ER). This probe exhibits exceptional temperature sensitivity of 3.9% per °C, with stable performance unaffected by pH or Ca2+ concentrations. It is ideal for real-time monitoring of local temperature changes in the ER, maintaining functionality in fixed cells. ER Tracker Yellow is compatible with a wide variety of cell lines and primary cells, including HeLa and MEF cells, making it a versatile tool for cellular research applications. -
Fluorescent Dye
Thiazole orange dimer YOYO 1 is a fluorescent dye that specifically targets DNA for staining applications. As a tetracationic homodimer of oxazole yellow, it exhibits minimal fluorescence in solution but significantly enhances its emission upon binding to double-stranded DNA via diintercalation, resulting in a fluorescence intensity increase of over 3200-fold. This remarkable amplification of fluorescence makes YOYO 1 an invaluable tool for various research applications, including nucleic acid quantification and visualization in molecular biology and genetic studies. -
Red Fluorophore
DFAME is a red fluorophore with excitation and emission wavelengths of 508 nm and 641 nm, respectively. It selectively binds to dimeric fluorescent RNA aptamers derived from Beetroot and Corn, yielding complexes with dissociation constants of 460 nM and 3600 nM, respectively. These Beetroot-DFAME and Corn-DFAME complexes facilitate the assembly of RNA structures within living cells, making them valuable tools for studying RNA nanostructures. The application of DNA/RNA nanostructures holds significant promise in advancing research in cell and gene therapy. -
Chemiluminescent Compound
Luminol sodium salt is a chemiluminescent compound that produces light at a maximum wavelength of 425 nm. This compound is widely utilized in forensic science as a sensitive indicator for the presence of blood stains, leveraging its unique ability to emit light in the presence of hemoglobin. Additionally, Luminol sodium salt has applications in environmental monitoring and biochemical assays, enhancing detection and analysis in various research fields. -
Fluorescent Dye
S0456 is a fluorescent dye precursor that facilitates the synthesis of the folate receptor-targeted near-infrared dye OTL 38. It enhances OTL 38 by imparting excellent optical properties along with high affinity and specificity for folate receptors. This compound is valuable in the research of tumor optical imaging agents, aiding in the development of advanced imaging techniques for cancer diagnostics. -
Quencher
Dabcyl acid is a nonfluorescent chromophore used primarily as a quencher in molecular fluorescent assays. It is effective in molecular beacon applications for the detection and reporting of specific nucleic acids in homogeneous solutions. Dabcyl acid enhances the sensitivity and specificity of nucleic acid probes, making it a valuable tool in genetic research and diagnostics. -
LDs/Mitochondria Dye
BETA-1 is a twisted intramolecular charge transfer (TICT) aggregation-induced emission (AIE) dye that targets lipid droplets (LDs) and mitochondria. It exhibits cyan fluorescence in LDs and red fluorescence in mitochondria, facilitating simultaneous dual-color imaging. BETA-1 is suitable for both in vivo and in vitro studies, making it an essential tool for researchers investigating cellular lipid metabolism and mitochondrial function. -
Hypochlorous Acid Detector
HKOCl-3 is a selective fluorescent probe designed for the detection of hypochlorous acid. This reagent exhibits high sensitivity with an excitation wavelength of 490 nm and an emission wavelength of 527 nm. HKOCl-3 is suitable for various biological research applications involving oxidative stress, inflammation studies, and the monitoring of hypochlorous acid levels in biological samples. -
Lipophilic Fluorescent Probe
5-Dodecanoylaminofluorescein is a lipophilic fluorescent probe that serves as a free fatty acid conjugate of fluorescein. This compound is instrumental in studies of membrane fluidity and the determination of critical micelle concentrations of detergents. Additionally, it can be utilized in the synthesis of hydrophobic nanospheres, enhancing applications in drug delivery systems. -
Fluorescent Calcium Indicator
Calcium Green 1AM is a cell-permeant fluorescent calcium indicator that targets calcium ions within cells. Upon cellular uptake, it is converted by intracellular esterases into its active form, facilitating precise measurement of calcium fluctuations. This reagent is widely utilized in cell physiology studies, calcium signaling research, and live-cell imaging applications. -
Fluorescent Dye
Acridine Orange base is a cell-permeable fluorescent dye primarily utilized for staining nucleic acids in various organisms, including bacteria, parasites, and viruses. This dye exhibits distinct fluorescence characteristics; it emits green light when bound to double-stranded DNA (Ex=488 nm, Em=520-524 nm) and red light when associated with single-stranded DNA or RNA (Ex=457 nm, Em=630-644 nm). Acridine Orange base is particularly valuable in fluorescence microscopy for distinguishing human cells under acidic conditions (pH=3.5) and is widely employed in cell cycle assays. -
Fluorescent Indicator
SBFI-AM is a sodium-selective fluorescent indicator designed for monitoring Na+ concentrations in biological systems. With a Kd of 6.7 mM for Na+ and 166 mM for K+, SBFI-AM demonstrates high selectivity for sodium ions. Its excitation and emission wavelengths are 340/380 nm and 500 nm, respectively. This reagent is widely used in cellular imaging and physiological studies to investigate sodium signaling and ion homeostasis. -
Lysosome-Specific Fluorescent Dye
Green DND-26 is a lysosome-specific fluorescent dye that exhibits a maximum excitation/emission wavelength of 504/511 nm. Comprising a fluorescein moiety linked to weak bases, this compound effectively penetrates cell membranes and accumulates in lysosomal structures. It is ideal for studying lysosomal biosynthesis and related pathological processes, facilitating the observation of cellular dynamics associated with lysosomal function. -
Neuronal Retrograde Tracer
True Blue is a fluorescent dye that serves as a neuronal retrograde tracer, excitable within the 395-425 nm wavelength range, with an emission barrier filter of 450 nm. It effectively labels neurons without compromising their survival, making it suitable for applications in neuronal tracing and mapping studies. This reagent is valuable for understanding neural connectivity and function in various research settings. -
DNA Dye
DAPI (4',6-Diamidino-2-phenylindole) is a fluorescent DNA dye that selectively binds to the AT base pairs in the minor groove of double-stranded DNA, enhancing fluorescence intensity approximately 20-fold upon binding. This property makes DAPI suitable for applications in fluorescence microscopy, allowing for quantification of DNA based on fluorescence intensity. Additionally, DAPI's ability to penetrate intact cell membranes enables its use in staining both live and fixed cells. DAPI also acts as an inhibitor of acid-sensing ion channel 3 (ASIC3), potentially contributing to research in chronic pain treatment (Ex/Em = 356/451 nm). -
Aluminum Ion Fluorescent Dyes
Lumogallion is a selective fluorescent reagent designed for the detection of aluminum ions, gallium, and other metal ions. Its high sensitivity allows for precise quantification and visualization in various biological and environmental samples. With an excitation wavelength of 490 nm and an emission spectrum ranging from 520 nm to 650 nm, Lumogallion exhibits a peak emission at approximately 580 nm, making it suitable for fluorescence-based applications in chemical research and analytical studies. -
Organic Anion Dye
Sulfobromophthalein disodium salt is an organic anion dye primarily utilized to investigate membrane carriers in animal tissues. This compound plays a critical role in understanding the transport mechanisms of various agents and metabolites across cell membranes. Its applications extend to studies related to pharmacokinetics and drug absorption, making it a valuable tool for researchers in the fields of biochemistry and pharmacology. -
Fluorescent Dye
DCVJ (9-(2,2-Dicyanovinyl)julolidine) is a unique fluorescent dye known for its ability to act as a molecular rotor. It exhibits significantly enhanced fluorescence intensity upon the polymerization of tubulin and actin, making it a valuable tool for studying cytoskeletal dynamics. Additionally, DCVJ interacts with phospholipid bilayers, further amplifying fluorescence. This compound is applicable in research involving mast cell degranulation, with optimal absorption and emission spectra at 489 nm and 505 nm, respectively. -
Fluorescent Imaging Agent
Pafolacianine is a near-infrared fluorescent imaging agent utilized for the detection of tumor lesions during surgical interventions. This compound operates by exciting at wavelengths of 774 to 775 nm and emitting at 794 to 796 nm, allowing for enhanced visualization of tumor tissues. Its application in intraoperative imaging significantly aids in improving surgical outcomes by providing real-time feedback for tumor localization. -
H2O2 Fluorescent Probe
HKPerox-2 is a highly sensitive fluorescent probe specifically designed for the detection of hydrogen peroxide (H2O2) in live cells. It exhibits peak excitation and emission wavelengths at 520 nm and 543 nm, respectively, making it suitable for fluorescence microscopy applications. This reagent can be utilized to study oxidative stress and cellular signaling involving reactive oxygen species, enabling insights into various biological processes and disease mechanisms. -
Fluorescent Dye
CellTracker Blue CMF2HC Dye is a fluorescent dye primarily used for visualizing and tracking live cells. It emits a blue fluorescence when excited at wavelengths between 450-460 nm, making it suitable for applications such as two-channel nucleic acid sequencing. Additionally, this dye facilitates the rapid assessment of microbial antibiotic sensitivity, providing valuable insights for microbiological research. -
Phospholipid Fluorescent Probe
BODIPY FL-DHPE is a green-fluorescent phospholipid probe targeting lipid bilayers. It labels 1,2-dihexadecanoyl-sn-glycero-phosphoethanolamine (DHPE), facilitating the study of membrane dynamics through membrane fusion processes. This reagent is ideal for investigating membrane structure and function in various cellular contexts, as well as analyzing lipid interactions in live cell imaging applications. The excitation and emission wavelengths are λex=505 nm and λem=511 nm, respectively. -
Cationic Carbocyanine Dye
Stains-All is a cationic carbocyanine dye that serves as an effective probe for investigating the structural characteristics of individual calcium-binding sites within calmodulin (CaM) and other related calcium-binding proteins (CaBP). This dye facilitates the study of calcium-binding interactions, making it an invaluable tool in cellular and molecular biology research focused on calcium signaling pathways and protein interactions. -
Oxidized Fluorescent Product
2',7'-Dichlorofluorescein is a potent fluorescent probe primarily used for the detection of oxidative stress by quantifying reactive oxygen species (ROS) levels. It exhibits excitation and emission maxima at 503 nm and 523 nm, respectively. This compound is essential for applications in cellular biology and biochemistry, enabling researchers to study oxidative damage and its effects on biological systems. -
OH Indicator
HKOH-1r is a highly sensitive green fluorescent probe designed for the selective detection of hydroxyl radicals (·OH) in living cells. It exhibits excitation and emission maxima at 500 nm and 520 nm, respectively, facilitating real-time visualization of oxidative stress. This reagent is applicable in various research settings, including studies focused on cellular redox status and oxidative damage pathways. -
Fluorescent Probe
DASPEI is a cationic styrenyl dye that targets mitochondria, functioning as a fluorescent probe with a large Stokes shift. Exhibiting excitation and emission wavelengths of 550 nm and 573 nm respectively, DASPEI demonstrates excellent photostability and labeling capability. This reagent effectively stains mitochondria in living cells and can also label presynaptic nerve endings, providing insights into cellular and synaptic activity. Its applications are valuable in various fields including neurobiology and cellular imaging research. -
Microenvironment-sensitive Membrane Reporter
Prodan is a solvatochromic fluorophore that serves as a microenvironment-sensitive membrane reporter. Its unique properties allow for the detection of changes in the local environment of membranes by varying Stokes shift values in response to the orientational polarizability of solvents. This versatility makes Prodan an essential tool in the study of membrane dynamics and the characterization of fluorescent nucleosides. It is particularly useful in applications involving membrane biophysics and the assessment of lipid bilayer properties. -
Fluorescent Dye
6-CFDA is a fluorescent dye commonly used in biological research for cell tracking and viability assays. It undergoes free diffusion into cells, where it is hydrolyzed by intracellular non-specific lipases to produce carboxyfluorescein, a fluorescent compound that carries an additional negative charge. This enhanced charge improves retention within cells compared to traditional fluorescein dyes, facilitating more accurate analysis of cellular processes and functions. -
Fluorescent Marker
6-ROX is a selective fluorescent probe targeting cyclooxygenase-2 (COX-2). By binding to the active site of COX-2, it inhibits the conversion of arachidonic acid to prostaglandins, making it an essential tool for studying COX-2-related pathways. 6-ROX is widely utilized in optical imaging applications aimed at identifying tumors and inflamed tissues that exhibit elevated COX-2 expression.
