Catalog No.
Product Name
Application
Product Information
Citations
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Fluorescent Probe
PS-SCOTfluor-12 is a fluorescent probe designed for the imaging of lactic acid metabolism in living cells. With an excitation wavelength of 485 nm and an emission wavelength of 605 nm, it allows for the visualization of metabolic processes in real-time. This reagent is particularly valuable for studies in cancer research and other fields where lactic acid metabolism plays a critical role. -
Peroxynitrite Fluorescent Probe
DMANI is a peroxynitrite fluorescent probe that employs an intramolecular charge transfer (ICT) mechanism for ratiometric detection. This probe selectively targets mitochondria, enabling the monitoring of mitochondrial peroxynitrite (ONOO−) levels in living cells. DMANI serves as a valuable tool for studying oxidative stress and related pathophysiological processes in cellular environments. -
DKK1 Inhibitor
Gallocyanine chloride is a DKK1 inhibitor that targets the DKK1/LRP6 interaction, exhibiting an IC50 of 6.38 μM. This compound is relevant for research into Alzheimer's disease and related neurodegenerative tauopathies, as it activates the Wnt signaling pathway and promotes β-catenin accumulation. Additionally, Gallocyanine chloride demonstrates anti-metastatic, anti-inflammatory, and anti-fibrotic properties, and can serve as a fluorescent probe for the detection of superoxide anion radicals. -
PH Indicator
Pyranine is a cell-impermeant, pH-sensitive fluorescent indicator that serves primarily as a pH monitor. This compound exhibits strong fluorescence and acts as a chemosensor for copper ions (Cu+), making it valuable for various biochemical applications. Pyranine is widely utilized as a biological stain, optical detecting reagent, and as a coloring agent in research environments, with an excitation/emission spectrum of 450/510 nm. Its ability to provide precise pH measurements enhances its utility in various experimental setups. -
Calcium Ion Indicator
Mag-Fluo-4 AM is a fluorescent calcium ion indicator that exhibits a high affinity for Ca²⁺. This compound enables the specific detection of intracellular calcium ions with exceptional sensitivity and minimal cytotoxicity. Upon entering the cell, it is hydrolyzed by intracellular esterases, allowing it to bind calcium ions and emit strong fluorescence. Mag-Fluo-4 AM is widely applicable in studies involving calcium signaling and cellular functions. -
Iron Dye Reagent
Ferrozine is an iron dye reagent that specifically targets divalent iron ions (Fe2+). Upon reaction, it forms a stable magenta complex, which exhibits a maximum absorption peak at 562 nm. This property enables the use of Ferrozine in colorimetric assays for the quantitation of iron in biological samples, facilitating research in iron metabolism and cellular iron homeostasis. -
Biochemical Assay Reagent
Bathocuproine disulfonate disodium (BCS) is a highly sensitive colorimetric reagent utilized for the detection of copper ions in biochemical assays. This compound exhibits a bright yellow color and possesses strong absorbance at specific wavelengths, enabling the quantification of trace levels of copper. BCS is instrumental in studying copper's biological roles, as well as in environmental monitoring and water quality testing, where its sensitivity allows for the identification of copper pollution, even at minimal concentrations. Accurate measurement of copper is essential for understanding its physiological effects and ecological impacts. -
Reference Dye
Diphenyl Blue, also known as Trypan Blue, is a vital reference dye primarily used to assess cell membrane integrity and viability. It selectively stains dead or damaged cells blue, allowing for the identification of non-viable cells in various tissue and cell culture applications. Viable cells with intact membranes exclude the dye, while macrophages can phagocytize Diphenyl Blue, making it an effective living stain for studying these immune cells. This dye is essential for research involving cell viability assays and tissue analyses. -
ROS Probe
Lucigenin is a chemiluminescence probe primarily utilized for the detection of the endogenous superoxide anion radical (O2-). This compound exhibits high sensitivity and can also function as a chloride indicator, as its fluorescence is quenched in the presence of chloride ions. With excitation and emission wavelengths of 455 nm and 505 nm, respectively, Lucigenin serves as a valuable tool in studies involving reactive oxygen species and chloride ion interactions. -
Lipophilic Fluorescent Dye
BODIPY 505/515 is a lipophilic fluorescent dye with a strong ultraviolet absorption capacity and sharp fluorescence peak, offering a high quantum yield. This compound exhibits stability across various physiological conditions and is relatively insensitive to environmental polarity and pH. BODIPY 505/515 effectively penetrates cell membranes, allowing specific labeling of neutral lipids within lipid droplets in both live and fixed cells. It is ideal for applications in cellular imaging, studying lipid metabolism, and analyzing cellular lipid storage. Maximum excitation and emission wavelengths are 505 nm and 515 nm, respectively. -
Fluorescent Probe
2′,7′-Dichlorofluorescein diacetate (DCFH2-DA) is a cell-permeable fluorescent probe utilized for the detection of reactive oxygen species (ROS). It becomes fluorescent upon cleavage by intracellular esterases and subsequent oxidation, allowing for the assessment of oxidative stress levels in biological systems. This reagent is essential for studies in toxicology and cellular signaling, enabling researchers to monitor and evaluate oxidative phenomena in various experimental contexts. -
Cu+ Probe
Copper Fluor-4 (CF4) is a highly selective fluorescent probe for Cu+ ions, utilizing a rhodol dye scaffold. With a Kd value of 2.9×10−13 M, it demonstrates exceptional selectivity for copper over competing metal ions such as zinc and iron, as well as common cellular alkali and alkaline earth metals. Copper Fluor-4 remains stable within a physiologically relevant pH range of 6 to 8, making it suitable for various biological applications, including imaging and quantification of copper ions in live cells. Excitation occurs at 415 nm with emission at 660 nm. -
pH Indicator Dye
Phenol Red is a pH indicator dye that exhibits a notable color transition from pink to yellow in response to changes in acidity. This property makes it essential for applications in cell culture and biological research, where monitoring pH levels is crucial for maintaining optimal conditions for cell viability and function. Phenol Red is widely utilized in laboratory assays and as a diagnostic tool for evaluating the conditions of various biological systems. -
Fluorescent Probe
MitoPerOx is a fluorescent probe designed to detect lipid peroxidation specifically in mitochondria, utilizing BODIPY581/591 fluorophores. With its triphenylphosphine cation (TPP+), MitoPerOx selectively accumulates in mitochondria based on membrane potential, allowing for sensitive measurement of lipid peroxides within the inner mitochondrial membrane. The probe exhibits a ratiometric emission shift from 590 nm to 520 nm upon oxidation, facilitating accurate detection at an excitation wavelength of 488 nm. MitoPerOx is valuable for investigating oxidative stress-associated diseases, including aging, neurodegenerative disorders, and mitochondrial dysfunction. -
Fluorescent Dye
Lucifer Yellow CH dipotassium salt functions as a high-intensity fluorescent dye, primarily utilized for its ability to react with fatty aldehydes at room temperature. This probe is valuable in biological research for tracing neuronal branching, monitoring regeneration, and detecting gap junctions, as well as for selective ablation of cells following aldehyde fixation. It exhibits optimal excitation and emission wavelengths of 430 nm and 540 nm, respectively, making it an effective tool in various fluorescence microscopy applications. -
Fluorescent Probe
Carboxy-SNARF 1 (5/6-mixture) is a pH-sensitive fluorescent probe utilized for the measurement of intracellular and environmental pH changes. It exhibits a significant emission shift from yellow-orange (Em: 580 nm) at acidic pH to deep red fluorescence (Em: 640 nm) in basic conditions, making it particularly useful for dynamic pH monitoring in biological systems. This probe is valuable in various research applications, including studying cellular metabolism, enzyme activity, and membrane potential. -
Lymphocyte Tracker
5-Carboxyfluorescein diacetate N-succinimidyl ester is a cell-permeable fluorescent dye with an excitation wavelength of 492 nm and an emission wavelength of 517 nm. It covalently labels intracellular proteins, enabling the tracking of lymphocyte migration and proliferation in biological research. This reagent is particularly useful for studying immune responses and cellular dynamics in various experimental settings. -
Chemical
1,8-Diazafluoren-9-one is a chemical reagent primarily utilized in forensic analysis for the detection of latent fingerprints on porous surfaces. This compound reacts with amino acids found in fingerprints, resulting in the formation of highly fluorescent derivatives, with an excitation/emission maximum of approximately 470/570 nm. Its applications extend to enhancing imaging techniques in forensic science, facilitating reliable identification in criminal investigations. -
Calcium Indicator
Fluo-3 AM is a fluorescent calcium indicator that effectively chelates Ca2+ ions with high affinity. It is designed for the sensitive detection of intracellular calcium fluctuations, making it a valuable tool for monitoring cellular calcium dynamics. Due to its low cytotoxicity and efficient cellular uptake facilitated by the acetoxymethyl ester moiety, Fluo-3 AM is cleaved intracellularly, allowing for sustained binding to calcium ions and robust fluorescence output. This compound is widely used in studies related to signal transduction, muscle contraction, and various calcium-dependent cellular processes. -
Fat-soluble Diazo Dye
Sudan Black B is a fat-soluble diazo dye used primarily as a histochemical stain. This reagent effectively stains neutral triglycerides and lipids, making it a valuable tool in the identification and analysis of adipose tissues and lipid-containing structures in biological samples. Its applications extend to research in lipid metabolism, pathology, and histological studies. -
Cell Proliferation Assay Reagent
WST-1 is a water-soluble tetrazolium salt that serves as a reagent for cell proliferation assays. It selectively undergoes reduction by intracellular mitochondrial dehydrogenases, resulting in the production of a soluble formazan product detectable at 450 nm. This compound is utilized to assess cell viability and cytotoxicity, making it an essential tool in various biological and pharmacological research applications. -
Golgi-specific Fluorescent Dye
BODIPY FL C5-Ceramide is a Golgi-specific fluorescent dye that targets the Golgi apparatus for precise cellular visualization. This BODIPY-labeled ceramide derivative is synthesized in the endoplasmic reticulum and is effectively transported to the Golgi through ceramide transport proteins or vesicular mechanisms. With an excitation/emission wavelength of 505 nm/512 nm, it is suited for applications in cell biology and imaging studies, allowing researchers to observe and analyze cellular structures with enhanced clarity. -
Supravital Stain
Janus Green B is a supravital stain utilized for the visualization of live cells and tissues. This compound exhibits an oxygen-dependent staining reaction that can be reversibly inhibited by cyanide, making it a valuable tool in live-cell imaging. It has been effectively employed in the staining of peripheral nerves in insects, lymphatic vessels in rabbits, and mitochondria, aiding in various biological research applications related to cellular morphology and function. -
Fluorescent Dye
4-Di-1-ASP is a styryl dye that targets glioma cells for fluorescent imaging in live brain tissue. This reagent enables detailed analysis of cellular structures, viability, proliferation, and endocytic processes, including cytokinesis and phagocytosis. Additionally, 4-Di-1-ASP facilitates observations of mitochondrial architecture in living cells, exhibiting green fluorescence upon excitation (λex /λem = 475/606 nm). -
Fluorescent Probe
BODIPY-C12 is a boron-dipyrromethene derivative functioning as a fluorescent probe with an excitation wavelength of 490 nm. Its hydrophobic structure allows it to be highly sensitive to its surrounding environment, making it suitable for quantifying viscosity, critical micelle concentration, and critical micelle temperature. This compound serves as a valuable tool for researchers in assessing lipid environment dynamics and micelle formation in various biological studies. -
Fluorescent Dye
Calcein (mixture of isomers) is a calcium-dependent fluorescent dye that enables the visualization of calcium ions in biological systems. This reagent is commonly utilized in studies of bone metabolism and is effective for staining specific cellular regions in vitro. Additionally, Calcein can be employed in fluorometric assays and for the titration of calcium ions using EDTA, making it a versatile tool for various research applications. -
Fluorescent Dye
3,3'-Dihexyloxacarbocyanine iodide is a carbocyanine fluorescent dye primarily used for the assessment of mitochondrial membrane potential. This dye exhibits significant fluorescence characteristics, making it an effective tool for studying mitochondrial dynamics and function in various cellular processes. It is widely utilized in research applications involving cell viability, apoptosis studies, and mitochondrial health assessments. -
pH Sensitive Fuorescent Probe
9-Amino-6-chloro-2-methoxyacridine is a pH-sensitive fluorescent probe that exhibits significant changes in fluorescence intensity in response to pH variations. This compound is particularly useful for monitoring vacuolar pH changes during substrate transport across the tonoplast via a proposed H+/solute antiport mechanism. Its application in cellular and molecular research facilitates the understanding of acid-base homeostasis in plant cells and other biological systems. -
Tumor Targeted Dye
MHI-148 is a near-infrared heptamethine cyanine dye that selectively targets tumors, making it ideal for cancer detection and diagnosis. This compound demonstrates preferential accumulation in the lysosomes and mitochondria of tumor cells, allowing for enhanced visualization. Its unique properties facilitate research applications in tumor biology and imaging studies, enabling more effective monitoring and understanding of cancer progression. -
Fluorescent Dye
2,3-Diaminonaphthalene is a selective fluorescent dye primarily used for the detection of selenium ions. It exhibits strong colorimetric and fluorometric properties, making it suitable for quantifying nitrite levels as well. This reagent finds applications in environmental monitoring and analytical chemistry, enabling sensitive detection and measurement of specific analytes in various research settings. -
Lipid Fluorescent Probe
NBD-PE is a lipid fluorescent probe that targets lipid bilayers, excelling in membrane and cell biology research. With an excitation wavelength of 465 nm and emission at 535 nm, this compound facilitates visualization of cellular membranes and lipid dynamics. Key applications include studying membrane structure, dynamics, and interactions in live cell imaging. -
Fluorescent Dye
BODIPY 500/510 C1, C12 is a fluorescent dye characterized by strong ultraviolet absorption and high quantum yield. It features a sharp fluorescence peak and remains stable across various physiological conditions, demonstrating minimal sensitivity to polarity and pH variations. This dye effectively penetrates cell membranes, allowing for the specific localization of neutral lipids within lipid droplets in both live and fixed cells. It is widely utilized for cellular imaging and lipid droplet labeling applications. Maximum excitation and emission wavelengths are 500 nm and 510 nm, respectively. Protect from light and store at -20°C. -
Fluorescent Membrane-staining Dye
Di-4-ANEPPDHQ is a polarity-sensitive membrane probe designed for visualizing membrane microdomains in living cells. This doubly charged fluorescent dye enables the assessment of lipid accumulation by producing a fluorescence spectrum shift in response to changes in the polarity of the lipid environment upon embedding within the cell membrane. With an excitation wavelength of 488 nm and emission wavelengths of 560 nm and 650 nm corresponding to liquid ordered and disordered phases, Di-4-ANEPPDHQ is ideal for fluorescence imaging studies focusing on membrane microstructure, lipid order, and membrane tension response. -
Amyloid-β Inhibitor
Hoechst 34580 tetrahydrochloride is a nuclear marker dye that selectively targets A/T-rich double-stranded DNA. This compound exhibits enhanced fluorescence intensity when bound to nucleic acids, making it valuable for live cell labeling applications. As the pH of the solution increases, the fluorescence intensity of Hoechst 34580 also increases, providing a reliable tool for studying cellular dynamics, DNA distribution, and nuclear morphology in real-time. This reagent is particularly useful in research focused on amyloid-β and related neurodegenerative processes. -
Fluorescent Probe
DAF-2DA (5,6-Diaminofluorescein diacetate) is a fluorescent probe designed for the selective detection of nitric oxide (NO) in biological samples. Upon reaction with NO, DAF-2DA is converted into a highly fluorescent compound, allowing for quantitative measurement of NO levels. This reagent is widely utilized in cellular and physiological studies to investigate NO signaling pathways and its role in various biological processes. -
H2O2 probe
Peroxy Orange 1 is a hydrogen peroxide (H2O2) specific probe designed for the detection of reactive oxygen species. It binds selectively to highly reactive oxygen species, enabling the visualization of H2O2 in live cells through green fluorescence. This compound is valuable for studying oxidative stress, cellular signaling, and related biological processes in various research applications. -
Cell Membrane Staining Agent
Octadecyl Rhodamine B chloride is a versatile amphiphilic fluorescent probe targeting cell membranes. This compound effectively stains cell membranes and is instrumental in membrane biology research. It exhibits fluorescence properties, with a maximum absorption and emission wavelength of 565 nm and 585 nm, respectively, when in Triton X-100. In aqueous solutions, the compound forms aggregates that shift absorption peaks to 524 nm and 574 nm, resulting in notable fluorescence quenching. -
Mitochondria Fluorescent Dye
MitoTracker Orange CMTMRos is a fluorescent dye that selectively labels mitochondria in live cells by exploiting the mitochondrial membrane potential (Ex/Em: 551/576 nm). This compound is essential for studying mitochondrial dynamics, morphology, and function. It is widely used in research applications, including cellular metabolism, apoptosis, and drug-response assays in various cell types. -
Zinc Ion Fluorophore
Zinquin ethyl ester is a zinc ion fluorophore specifically designed for the detection of intracellular zinc ions. This lipophilic probe readily penetrates cell membranes and exhibits a strong affinity for Zn2+, resulting in blue fluorescence upon binding. Primarily utilized in research applications focused on zinc signaling and cellular metal ion dynamics, Zinquin ethyl ester serves as a valuable tool for studying zinc-related biological processes. -
Lipophilic Fluorochrome
CellTracker CM-DiI is a lipophilic fluorochrome designed for the visualization and tracking of lymphocyte migration. This reagent enables the labeling of cells for detection in histological sections, with an excitation wavelength of 553 nm and an emission wavelength of 570 nm. Its application is critical in studies examining cellular dynamics and immune responses. -
Fluorogenic Ligand
DFHO is a fluorogenic ligand specifically designed for binding with the Corn fluorogenic aptamer. With a Kd value of 70 nM, DFHO undergoes a conformational change upon binding, resulting in enhanced fluorescence, thereby facilitating RNA imaging within cellular environments. This compound is valuable for researchers investigating RNA dynamics and localization in live cell assays. -
Mitochondrial ROS Indicator
MitoSOX Red is a live cell fluorescent probe that specifically targets mitochondria and is cell membrane permeable. MitoSOX Red enters mitochondria and is oxidized by superoxide but not by other ROS or RNS generating systems. The oxidized MitoSOX Red then binds to nucleic acids in mitochondria/nucleus, producing strong red fluorescence. MitoSOX Red can be used as a fluorescent indicator to specifically detect superoxide. In addition, superoxide dismutase (SOD) can prevent the oxidation of MitoSOX Red. Excitation/emission wavelength: 510/580 nm. -
Hydrophobic Fluorescent Stain
Nile red (Nile blue oxazone) is a lipophilic stain. Nile red has environment-sensitive fluorescence. Nile red is intensely fluorescent in a lipid-rich environment while it has minimal fluorescence in aqueous media. Nile red is an excellent vital stain for the detection of intracellular lipid droplets by fluorescence microscopy and flow cytof uorometry. Nile red stains intracellular lipid droplets red. The fluorescence wavelength is 559/635 nm. -
Fluorochrome
Propidium Iodide (PI) is a nuclear staining agent that stains DNA. Propidium Iodide is an analogue of ethidine bromide that emits red fluorescence upon embedding in double-stranded DNA. Propidium Iodide cannot pass through living cell membranes, but it can pass through damaged cell membranes to stain the nucleus. Propidium Iodide has a fluorescence wavelength of 493/617 nm and a wavelength of 536/635 nm after Mosaic with DNA. Propidium Iodide is commonly used in the detection of apoptosis (apoptosis) or necrosis (necrosis), and is often used in flow cytometry analysis. -
Green Dye
BODIPY493/503 methyl bromide is a BODIPY dye. BODIPY dye is a small molecule dye with strong ultraviolet absorption ability, its fluorescence peak is relatively sharp, and the quantum yield is high. They are relatively insensitive to the polarity and pH of the environment and are relatively stable under different physiological conditions. Due to its structural asymmetry, BODIPY derives a variety of structural products. BODIPY lipid droplet dyes can well pass through the cell membrane into the cell, and localize the neutral lipids in the cell to specifically stain the lipid droplets, which can be used for labeling of live cells and fixed cells. Maximum excitation/emission wavelength: 493/503 nm. -
Neutral Lipid Dye
BODIPY 493/503 is a member of the BODIPY (boron-dipyrromethene) family of small-molecule fluorescent dyes, characterized by strong absorption in the ultraviolet-visible range, narrow and well-defined emission spectra, and high fluorescence quantum yields. These dyes exhibit minimal sensitivity to environmental polarity and pH, maintaining stable photophysical properties under a wide range of physiological conditions.
Due to the structural versatility of the BODIPY core, numerous derivatives with tailored properties can be generated. BODIPY-based lipid droplet probes readily permeate cellular membranes and selectively localize to neutral lipid compartments, enabling specific staining of intracellular lipid droplets. This makes them suitable for both live-cell imaging and fixed-cell applications.
Maximum excitation/emission wavelengths: 493/503 nm.
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Voltage-Sensitive Dye
Di-4-ANEPPS is a voltage-sensitive dye that targets voltage-gated ion channels, primarily inhibiting sodium currents. This compound alters fluorescence properties in response to changes in membrane potential, facilitating the examination of ion channel function. It is useful in cardiovascular research, particularly in studies involving electrophysiology of cardiomyocytes, myocardial ischemia, and investigating drug effects on cardiac cells. Di-4-ANEPPS is instrumental in assessing drug cardiotoxicity and elucidating the mechanisms underlying arrhythmias. -
Anti-microbial Agent/Kir3.2 Blocker
3,6-Diaminoacridine hemisulfate is an acridine compound that functions primarily as a broad-spectrum antimicrobial agent and a Kir3.2 potassium channel blocker. Its mechanism involves insertion into bacterial DNA, thereby disrupting replication and transcription, which ultimately leads to bacterial lysis. Additionally, this compound is utilized in research to investigate the neurological phenotype associated with Down syndrome. Due to its ability to penetrate the stratum corneum and accumulate in the cell nucleus, prolonged exposure warrants caution due to potential oncogenic effects. -
Fluorescent Tracer
Fluorescein is a fluorescent tracer commonly employed in medicinal and biological research, particularly for visualizing tumor-infected tissues. As a representative green fluorophore, it serves as a versatile scaffold for the development of various green fluorescent probes. Its high solubility and brightness make it an excellent choice for applications in microscopy and flow cytometry, facilitating studies in cellular and molecular biology. -
ROS Probe
HKOH-1 is a sensitive green fluorescent probe designed for the selective detection of hydroxyl radicals (·OH) in living cells, utilizing a maximum excitation wavelength of 500 nm and an emission wavelength of 520 nm. This reagent enables researchers to monitor oxidative stress and assess cellular responses to reactive oxygen species. Its application is critical in studies related to redox biology, neurodegenerative diseases, and cancer research.
