Click Chemistry

Cyanine 3 DBCO chloride is a fluorescent lipid labeling reagent that incorporates a cycloalkyne group for click chemistry applications. This compound features a DBCO moiety, facilitating copper-free biocompatible click reactions with rapid kinetics and excellent stability. With excitation and emission wavelengths of 550 nm and 570 nm respectively, it is ideal for labeling and visualizing biomolecules in various biological research applications, including tracking cellular processes and studying lipid interactions.

Cyanine 3.5 azide is a fluorescent dye featuring an azide functional group, making it suitable for click chemistry applications. It effectively participates in copper-catalyzed azide-alkyne cycloaddition (CuAAc) reactions with alkyne-containing compounds, as well as in strain-promoted alkyne-azide cycloaddition (SPAAC) with DBCO or BCN groups. This versatile reagent exhibits excitation and emission maxima at 591 nm and 604 nm, respectively, and is ideal for labeling and imaging studies in chemical biology and biomolecular research.

(E)-2-Hexadecenal alkyne is a fluorescent dye that serves as a chemical probe to investigate the cellular targets of 2-trans-Hexadecenal. This compound covalently modifies a range of proteins within cells, making it an effective tool for studying diseases associated with lipid metabolism. Researchers can utilize this reagent to gain insights into protein interactions and signaling pathways influenced by lipid-derived compounds.

N'-Phenylacetohydrazide is a hydrazine-derived compound utilized primarily as a biochemical assay reagent. It selectively reacts with carbonyl groups, making it effective for the identification and quantification of aldehydes and ketones in various organic compounds. This versatile reagent is widely employed in organic chemistry for analytical purposes, facilitating research into carbonyl-containing substances.

BODIPY FL azide is a BDP fluorescent dye characterized by an azide group that enables Click Chemistry. This green fluorophore, belonging to the borodipyrromethane class, exhibits a high quantum yield in aqueous solutions and demonstrates excellent stability against photobleaching. It is particularly suitable for applications requiring FAM fluorescence measurements, making it an ideal tool for various biochemical assays and cellular imaging studies.

SM6.1-azide is a ligand specifically targeting the αvβ6 integrin, featuring an azide group. This reactive moiety enables click chemistry with small molecules, peptides, or siRNA that possess DBCO or alkyne functionalities, facilitating efficient pulmonary-targeted delivery. This compound is valuable for research applications focused on integrin interactions and targeted therapeutics in respiratory conditions.

Benzylhydrochlorothiazide-d5 is a deuterated analog of Benzylhydrochlorothiazide, serving as a stable isotope labeled compound. This reagent is primarily utilized in quantitative analysis, facilitating studies in pharmacokinetics and metabolism of thiazide diuretics. Its stable isotope nature allows for precise tracking and differentiation in biological assays and isotope labeling experiments.

BP Fluor 488 Alkyne is a fluorescent dye specifically designed for labeling in Click Chemistry reactions. It conjugates effectively with azide-modified biomolecules, including antibodies, proteins, peptides, and amino-modified oligonucleotides. With an excitation peak at 499 nm and emission peak at 520 nm, this dye allows for enhanced visualization in applications such as microscopy and flow cytometry. BP Fluor 488 Alkyne is a pure 5-sulfonated rhodamine derivative, providing consistent results free from lot-to-lot variation.

MB 543 DBCO is a fluorescent dye featuring a terminal DBCO group, which enables its application in copper-free Click Chemistry reactions through strain-promoted cycloaddition. This highly hydrophilic, water-soluble compound exhibits strong orange fluorescence with a maximum absorption at 543 nm and emission at 566 nm. Additionally, MB 543 DBCO demonstrates excellent stability across a broad pH range from 3 to 10, making it suitable for various biological imaging and labeling applications in research.

BP Fluor 350 Azide is a blue-fluorescent dye designed for bioorthogonal labeling, functioning primarily through copper-catalyzed azide-alkyne cycloaddition (CuAAC) and copper-free click chemistry with strained cyclooctynes to form stable triazole compounds. This water-soluble, moderately photostable probe is optimally excited at 350 nm and is ideal for applications in imaging and flow cytometry, providing robust signal generation for high-abundance target visualization. BP Fluor 350 is particularly suitable for experiments requiring direct imaging due to its outstanding brightness and stability.

BP Fluor 430 Picolyl Azide is a specialized fluorescent dye designed for enhanced signal amplification through its unique copper-chelating motif. This compound effectively increases the local concentration of Cu(I) at the reaction site, significantly accelerating the copper-catalyzed azide-alkyne cycloaddition (CuAAC) reaction without the need to elevate the concentrations of azide or copper catalysts. Utilizing BP Fluor 430 Picolyl Azide allows for at least a tenfold reduction in copper catalyst concentration while maintaining optimal labeling efficiency, making it ideal for the detection of low-abundance targets or applications necessitating increased signal intensity.

BP Fluor 488 Azide is a multifunctional fluorescent dye that primarily targets cellular structures and biomolecules. This dye plays a crucial role in biological research by enabling the visualization and tracking of cell dynamics, facilitating the evaluation of cellular functions, and enabling the differentiation of cell types. Its versatile applications extend across fundamental research and clinical diagnostics, as well as in textile processing, food coloring, and advanced technologies such as dye-sensitized solar cells.

BP Fluor 430 Azide is a water-soluble, green-fluorescent azide probe that targets terminal alkynes through copper-catalyzed azide-alkyne cycloaddition (CuAAC) and strained cyclooctynes via copper-free click chemistry, forming stable triazole compounds. It exhibits strong fluorescent properties with an excitation maximum at 432 nm and an emission maximum at 539 nm, demonstrating excellent brightness and photostability. This versatile probe remains pH-insensitive across a range of 4 to 10, making it suitable for a variety of biological applications, including the detection of low-abundance alkyne-tagged biomolecules.

BP Fluor 350 Picolyl Azide is a blue-fluorescent azide-activated probe designed for efficient labeling of biomolecules via copper-catalyzed azide-alkyne cycloaddition (CuAAC). Additionally, it can react with strained cyclic alkynes through copper-free click chemistry to form stable triazole linkages. This compound is valuable for fluorescence imaging and in studies requiring precise biomolecular tagging, allowing researchers to visualize and track interactions in various biological systems.

Rhodamine B carbohydrazide is a fluorescent dye that utilizes the spirocyclic structure of Rhodamine B to target specific reactive species. This compound exhibits strong fluorescence and is applicable for the detection of copper ions (Cu2+), mercury ions, peroxynitrite, hydroxyl radicals, and nitric oxide (NO). Its utility in biological research includes fluorescence imaging and monitoring oxidative stress, making it valuable for studies in environmental monitoring and cellular processes.

Cy3 hydrazide is a cyanine dye linker designed for the selective labeling of carbonyl-containing molecules, including aldehydes and ketones. This reagent effectively labels proteins such as antibodies and glycoproteins following periodate oxidation, as well as proteins affected by oxidative stress or deamination. Cy3 hydrazide is suitable for various fluorescent applications and is compatible with a wide range of fluorescence instrumentation, facilitating advanced biochemical and cellular studies.

FAM tetrazine, 5-isomer is a fluorescent dye characterized by its terminal tetrazine group, facilitating rapid click chemistry reactions with trans-cyclooctene (TCO). This compound serves as a versatile labeling agent in various biological applications, including protein tagging, cellular imaging, and detection of biomolecular interactions. Its high fluorescence and effective reactivity make it a valuable tool for researchers in fields such as biochemistry and molecular biology.

BP Fluor 350 Azide Plus is a fluorescent dye characterized by its terminal azide group. This compound exhibits maximum absorption at 340 nm and emission at 440 nm, making it suitable for various imaging applications. The incorporation of a copper-chelating system facilitates the formation of robust copper complexes, serving as both reactants and catalysts in copper-catalyzed azide-alkyne cycloaddition (CuAAC) reactions. It is ideally suited for studies requiring efficient labeling and detection in biological systems.

BP Fluor 555 Azide is a fluorescent dye designed for the labeling of biomolecules through copper-catalyzed or copper-free click chemistry. Exhibiting bright orange fluorescence, it has optimal excitation wavelengths of 532 nm and 555 nm, making it suitable for imaging applications with TRITC filter sets. The dye demonstrates pH insensitivity from pH 4 to pH 10, facilitating the detection of low-abundance targets when conjugated to antibodies, peptides, or proteins. Its high brightness and photostability enhance the visibility and reliability of fluorescent imaging techniques in various biological research applications.

BP Fluor 430 Alkyne is a green-fluorescent alkyne-activated probe designed for the imaging of azide-containing biomolecules with moderate to high abundance. It utilizes a copper-catalyzed azide-alkyne cycloaddition (CuAAC) reaction to produce a stable triazole linker. This probe exhibits bright and photostable fluorescence, optimally excited near 430 nm, and is water-soluble, maintaining pH-independent fluorescence across a wide range. Applications include cellular imaging and visualization of biomolecular interactions.

MB 660R DBCO is a far-red fluorescent dye, exhibiting strong fluorescence emission at approximately 685 nm. With an absorption maximum at around 665 nm, it can be effectively excited using 633 or 635 nm lasers. This dye is characterized by high water solubility and maintains stability across a broad pH range from 4 to 10. Its robust photostability and brightness make MB 660R DBCO suitable for various applications in fluorescent imaging and labeling in biological research.

BP Fluor 405 DBCO is a blue-fluorescent dye that utilizes copper-free click chemistry for targeted labeling of azides, resulting in stable triazole formation. Its excitation wavelength is optimal for use with the 407 nm spectral line of the krypton laser or the 408 nm violet laser diode. This dye is particularly useful in multi-color applications such as flow cytometry and super-resolution microscopy techniques like STORM, enabling precise visualization in complex biological samples.

BP Fluor 594 alkyne is a fluorescent dye designed for the specific imaging of azide-containing biomolecules. This probe utilizes a copper-catalyzed azide-alkyne cycloaddition (CuAAC) mechanism to react with azides, resulting in the formation of a stable triazole linkage. Its bright red fluorescence makes it an effective tool for various biological research applications, particularly in tracking biomolecular interactions and localization studies.

R6G azide, 5-isomer is a xanthene-derived fluorescent dye characterized by a terminal azide group. This compound can undergo Click Chemistry with alkynes, BCN, and DBCO, forming a stable triazole linkage. Its unique structure and fluorescent properties make it suitable for a range of applications, including bioimaging and labelling studies in chemical research.

BP Fluor 532 Azide Plus is a fluorescent dye featuring a terminal azide group, primarily used in click chemistry applications. With a maximum absorption at 532 nm and emission at 554 nm, this compound exhibits robust fluorescence properties. Its unique structure incorporates a copper-chelating system, facilitating the formation of stable copper complexes that function as both reactants and catalysts in CuAAC (Copper-catalyzed Azide-Alkyne Cycloaddition) reactions, making it ideal for a variety of biological and chemical research applications.

BP Fluor 647 Alkyne is a fluorescent dye characterized by its bright green fluorescence and optimal excitation with 633 and 650 nm Argon lasers. The alkyne moiety enables efficient reactions with azides through copper-catalyzed Click Chemistry. With its four sulfonate groups, this dye demonstrates excellent water solubility and reduced aggregation across a pH range of 4 to 10. BP Fluor 647 Alkyne is ideal for applications in protein and antibody labeling, as well as nucleic acid studies, allowing for high labeling density.

Tetra-sulfo-Cy7 DBCO is a highly fluorescent near-infrared dye designed for applications requiring low background noise and high sensitivity. This water-soluble, hydrophilic probe exhibits remarkable photostability and maintains consistent fluorescence across a pH range of 4 to 10, minimizing autofluorescence interference in biological samples. Tetra-sulfo-Cy7 DBCO is ideal for use in various imaging techniques, where its long-wavelength emission is optimized for detection by advanced imaging systems.

BP Fluor 488 Tetrazine is a bright green-fluorescent dye that serves as a highly effective probe for detecting TCO-tagged biopolymers. Its remarkable cycloaddition kinetics, reaching up to 30,000 M-1 s-1 with trans-cyclooctenes (TCO), make it the fastest reported for any bioorthogonal reaction. This compound is particularly valuable for applications requiring rapid reaction kinetics, such as in vivo cancer imaging and pre-targeted cell labeling studies.

BP Fluor 405 alkyne triTEA is a blue-fluorescent dye that serves as a versatile labeling reagent in multi-color applications, including flow cytometry and super-resolution microscopy techniques like STORM. It exhibits optimal excitation around the 407 nm spectral line of krypton lasers and the 408 nm violet laser diode. This dye participates in copper-catalyzed azide-alkyne cycloaddition (CuAAC), yielding a stable triazole linker upon reaction with azides. Additionally, a copper-free alternative, BP Fluor 405 DBCO, is available for applications sensitive to copper presence.

ROX azide, 5-isomer is a red-emitting fluorescent dye known for its high brightness and fluorescence quantum yield. The presence of an azide group enables reactive conjugation with alkyne, BCN, and DBCO through Click Chemistry, resulting in a stable triazole linkage. This compound is widely utilized in cellular imaging and labeling applications, facilitating the study of biomolecular interactions and dynamics.

BP Fluor 430 DBCO is a fluorescent dye that reacts with azides through a copper-free click chemistry mechanism, resulting in stable triazole formations without the need for copper catalysts or elevated temperatures. This dye exhibits bright, photostable green fluorescence, with optimal excitation at 432 nm and an emission peak at 539 nm, remaining pH independent across a broad pH range. BP Fluor 430 DBCO is suitable for various labeling applications in bioimaging and bioconjugation, although it is not recommended for staining intracellular components of fixed and permeabilized cells due to potential high background fluorescence.

BP Fluor 405 Picolyl Azide is a fluorescent dye designed for enhanced detection in bioconjugation reactions, utilizing a copper-chelating motif to increase local Cu(I) concentrations. This innovative reagent significantly accelerates the copper-catalyzed azide-alkyne cycloaddition (CuAAC) reaction rate while minimizing the required copper catalyst concentration by at least tenfold. The incorporation of the picolyl azide moiety allows for greater sensitivity in alkyne detection, making it particularly advantageous for applications involving low-abundance targets or those requiring amplified signal intensity. This makes BP Fluor 405 an essential tool for researchers focusing on advanced fluorescent labeling techniques.

Altizide is an orally active diuretic that primarily targets renal processes to promote increased urine output. It is effective in the management of edema and hypertension, making it a valuable tool in cardiovascular and renal research. Altizide can be employed in studies aimed at understanding fluid balance and electrolyte regulation.

DBCO-PEG2-OH is a functionalized polyethylene glycol (PEG) derivative designed for the selective labeling of azide-modified biomolecules through a click chemistry mechanism. This compound facilitates bioconjugation and is widely utilized in the development of drug carriers and in protein labeling applications. Its versatility makes it an important tool in biochemistry and chemical biology research.

L-Galactose-azide is an L-fucose analogue that demonstrates utility in glycosylation studies and carbohydrate chemistry. Its azide functional group allows for click chemistry applications, facilitating the development of glycosylated compounds. This reagent is particularly valuable in the field of glycobiology for probing interactions involving fucose-containing glycans.

Thiophosphoro alkyne dichloridate is a histidine coupling agent that functions as a thio-phosphonyl dichloride reagent. It enables selective covalent coupling with histidine residues, facilitating the development of protein function probes. This compound is particularly useful in studies related to protein phosphorylation and the exploration of histidine-based modifications in biological systems.

Docosahexaenoic acid alkyne is a derivative of docosahexaenoic acid (DHA) characterized by the presence of a terminal ω-alkyne functional group. This compound serves as a valuable tool for studying lipid metabolism and fatty acid signaling pathways in various biological systems. It can be utilized in click chemistry applications, enabling the labeling and visualization of DHA-containing molecules in cellular environments.

Buthiazide is a diuretic compound primarily targeting renal function to promote urine production. It is commonly utilized in research focused on hypertension and fluid regulation. This compound facilitates the study of electrolyte balance and its therapeutic implications in cardiovascular disorders.