Limited U937 growth inhibition and attachment, with antagonism of PMA
Cell-based study · Human U937 monocytic leukemia cells [1]
Over 60 h, bryostatin 1 caused much less growth inhibition and cell attachment than PMA. Its small response was biphasic. Co-treatment with bryostatin 1 opposed PMA-induced growth inhibition and attachment. Five independent experiments support the plotted growth and attachment data; results are specific to this cell model and schedule.
| Endpoint | Reported result | Reference |
|---|---|---|
| Total attached plus floating cells, relative to vehicle control | Cell number relative to DMSO Limited, biphasic growth inhibition with bryostatin 1 alone; suppression of PMA-induced growth inhibition with co-treatment | [1] |
| Attached cells as a percentage of total cells | Attachment Much less attachment with bryostatin 1 than PMA; co-treatment opposed PMA-induced attachment | [1] |
Literature-Reported Treatment Conditions
- Treatment concentration
- Bryostatin 1: 0.1–1000 nM concentration series; PMA comparator: 0.1–1000 nM.
- Exposure duration
- 60 h
- Method
- Treatment 24 h after plating; count attached and floating cells.
- Vehicle
- 0.1% DMSO in each sample
- Co-treatment
- Separate co-treatment arm: 10 nM PMA + 1000 nM bryostatin 1. Single-agent effects are reported separately.
- Reported limits
- No fitted growth-inhibition IC50 reported for the selected bryostatin 1 response. No Adooq lot tested.
Methods and source locations
Compound reported in the paper: Bryostatin 1; Compound label in the paper: bryostatin 1
Reported bryostatin 1 experiments; supplier and lot equivalence to Adooq A10261 were not established. No salt conversion or stereoisomer substitution is made.
Figure 2A–B and caption; Results: Biological Responses in U937 Leukemia Cells (text mis-cites Figure 1A–B); Materials; The growth and attachment of U937 cells
Total attached plus floating cells, relative to vehicle control: Beckman particle/Coulter counting after 60 h; plotted mean ± SEM, five independent experiments. No exact percentage inferred from bars. — Figure 2A and caption; The growth and attachment of U937 cells
Attached cells as a percentage of total cells: Attached cells counted after trypsinization and combined with floating-cell counts; 60 h treatment; plotted mean ± SEM, five independent experiments. — Figure 2B and caption; The growth and attachment of U937 cells