CCT241533 was reported to reduce BQ expression in LCC2 and AK-47 cells. In MCF-7 cells, the reported result for KU and CCT was no significant effect on H2AX or RPA2 phosphorylation or intracellular ERα content. In HFFF2 cells, CCT241533 suppressed TGFβ1-dependent SMA and FnEDA expression compared with TGFβ1-treated samples. [1,2,3]
Literature-reported observations and conditions; not Adooq-tested protocols or general treatment recommendations.
BQ expression in LCC2 cells
Cell-based study · LCC2 [1]
LCC2 cells were treated with CCT241533 at 10 nM for 72 h. CCT241533 was reported to reduce BQ expression, assessed by Western blot.
Literature-Reported Treatment Conditions
- Treatment concentration
- 10 nM
- Exposure duration
- 72 h
- Vehicle
- DMSO
- Reported limits
- Not stated in the sentences used: route, control.
Methods and source locations
Compound reported in the paper: CCT241533
Literature evidence for the named compound in the stated model; no Adooq product-use or lot-performance claim.
2. Results > 2.2. Suppression of CHK2 Activity Could Reduce Expression of BQ and Thus Tamoxifen Resistance (S40); 4. Materials and Methods > 4.6. Chemicals (S120); Figure 5 legend (S209, S210)
Western blot was employed to determine BQ expression.: Western blot was employed to determine BQ expression. — 2. Results > 2.2. Suppression of CHK2 Activity Could Reduce Expression of BQ and Thus Tamoxifen Resistance (S40); Figure 5 legend (S210)
Phosphorylation and intracellular ERα content in MCF-7 cells
Cell-based study · MCF-7 [2]
In MCF-7 cells assessed after 24 h, KU and CCT did not significantly affect H2AX or RPA2 phosphorylation or intracellular ERα content.
Literature-Reported Treatment Conditions
- Exposure duration
- 24 h
- Reported limits
- Not stated in the sentences used: dose, route, vehicle.
Methods and source locations
Compound reported in the paper: CCT241533
Literature evidence for the named compound in the stated model; no Adooq product-use or lot-performance claim.
Results > The ATR:CHK1-dependent control of ERα intracellular concentration (S211, S212, S214)
phosphorylated γH2AX expression levels: phosphorylated γH2AX expression levels; comparator: control (0) — Results > The ATR:CHK1-dependent control of ERα intracellular concentration (S211, S212)
Treatment suppresses SMA and FnEDA expression in HFFF2 cells
Cell-based study · HFFF2 [3]
In HFFF2 cells, the compound was added at 1.5 μmol/L for 72 hours. Western blotting and quantification showed suppression of TGFβ1-dependent SMA and FnEDA expression compared with TGFβ1-treated samples.
Literature-Reported Treatment Conditions
- Treatment concentration
- 1.5 μmol/L
- Exposure duration
- for 72 hours
- Method
- added to the cells
- Vehicle
- DMSO
- Co-treatment
- Other interventions in this design: TGFβ1 2 ng/mL. Compound effect isolated by the comparison: TGFβ1-treated samples.
Methods and source locations
Compound reported in the paper: CCT241533
Literature evidence for the named compound in the stated model; no Adooq product-use or lot-performance claim.
Materials and Methods > Cell culture (S32, S34, S35); Results > Targeting ATM inhibits myofibroblast differentiation (S146); Figure 2. legend (S230, S233)
Western blotting and quantification: Western blotting and quantification; comparator: TGFβ1-treated samples — Results > Targeting ATM inhibits myofibroblast differentiation (S146); Figure 2. legend (S230)
BQ expression in AK-47 cells
Cell-based study · AK-47 [1]
AK-47 cells were treated with CCT241533 at 10 nM for 72 h. CCT241533 was reported to reduce BQ expression, assessed by Western blot.
Literature-Reported Treatment Conditions
- Treatment concentration
- 10 nM
- Exposure duration
- 72 h
- Vehicle
- DMSO
- Reported limits
- Not stated in the sentences used: route, control.
Methods and source locations
Compound reported in the paper: CCT241533
Literature evidence for the named compound in the stated model; no Adooq product-use or lot-performance claim.
2. Results > 2.2. Suppression of CHK2 Activity Could Reduce Expression of BQ and Thus Tamoxifen Resistance (S40); 4. Materials and Methods > 4.6. Chemicals (S120); Figure 5 legend (S209, S210)
Western blot was employed to determine BQ expression.: Western blot was employed to determine BQ expression. — 2. Results > 2.2. Suppression of CHK2 Activity Could Reduce Expression of BQ and Thus Tamoxifen Resistance (S40); Figure 5 legend (S210)
