Annexin-V apoptosis response across four breast cancer cell lines
Cell-based study · Human BT-474, T47D, MDA-MB-415 and CAMA-1 breast cancer cells [1]
EHT1864 increased Annexin-V-positive apoptosis in all four lines at 20 and 50 μM after 72 h, with a larger response in BT-474 and T47D. These are cellular treatment concentrations, not biochemical IC50 values. Pharmacological response alone does not prove exclusive Rac dependence.
| Endpoint | Reported result | Reference |
|---|---|---|
| Annexin-V-positive cells, including floating and adherent populations | apoptosis response Dose-related increases in Annexin-V-positive cells across four lines; Figure 1D caption defines *p < 0.0001 by Bonferroni post-hoc test vs. control unless bracketed. No dose-specific significance assignment or percentage estimated here. | [1] |
Literature-Reported Treatment Conditions
- Treatment concentration
- 0, 20 or 50 μM EHT1864 (Figure 1D)
- Exposure duration
- 72 h
- Method
- Cells cultured in DMEM with 10% FBS; floating and trypsin-displaced adherent cells analyzed together
- Co-treatment
- No cotreatment assigned to the EHT1864 dose-response groups. Separate assay-control wells received 5 μM BKM120 during the final 12 h; negative controls did not.
- Reported limits
- Vehicle and number of independent biological replicates/error-bar definition for Figure 1D are not specified in its caption or selected apoptosis methods; no values or n inferred.
Methods and source locations
Compound reported in the paper: EHT1864; Compound label in the paper: Diaxonhit-provided EHT1864; salt form unspecified
Cellular EHT1864 response in these four lines, without catalog-lot, salt-specific, clinical or universal Rac-selectivity claims.
Figure 1D and caption; Results first subsection; Methods: Cell lines and reagents, Apoptosis assay, Statistical analyses
Annexin-V-positive cells, including floating and adherent populations: ApoScreen Annexin Apoptosis kit with flow cytometry; ANOVA and Bonferroni comparison adjustment; no numerical percentages transcribed from bars — Figure 1D and caption; Results first subsection; Methods: Cell lines and reagents, Apoptosis assay, Statistical analyses