Fludarabine response in malignant and non-malignant mesothelial cell models
Cell-based study · Mero-14, Mero-25, IST-Mes2, NCI-H28 and MSTO-211H mesothelioma cells; non-malignant MeT-5A, LP-9 and HMC7 mesothelial models [1]
Fludarabine reduced the MTT proliferation-associated signal in the five malignant lines. The non-malignant models showed no reported proliferation reduction under the represented conditions. MTT is a metabolic surrogate, not a direct cell count or clinical safety test.
| Endpoint | Reported result | Reference |
|---|---|---|
| Proliferation-associated MTT signal versus vehicle | Qualitative time-course response Reduced in all five MPM lines with 1 μM F-araA; no reported reduction in MeT-5A at the tested 1 and 10 μM conditions. | [1] |
| MTT signal in additional non-malignant mesothelial models | Qualitative time-course response LP-9 and HMC7 proliferation was not affected by 1 μM F-araA in this assay. | [1] |
Literature-Reported Treatment Conditions
- Treatment concentration
- 1 and 10 μM for Figure 2A; 1 μM for Figure 3A–B
- Exposure duration
- 24, 48 and 72 h after treatment; pretreatment baseline shown
- Method
- Cell treatment 24 h after seeding; MTT time course
- Co-treatment
- Single-agent fludarabine; risedronic acid panels not selected.
- Reported limits
- Main text does not identify the specific solvent or final vehicle fraction for these MTT panels; matched vehicle controls were used. No IC50 calculated here.
Methods and source locations
Compound reported in the paper: Fludarabine (F-araA); Compound label in the paper: Fludarabine (F-araA)
Compound-level cellular evidence; does not establish analytical identity, potency or performance of an A10396 lot.
Figure 2A; Figure 3A–B; MTT assay; Chemicals; Statistical analysis
Proliferation-associated MTT signal versus vehicle: MTT optical density; Figure 2: mean ± SEM, three independent experiments, each in triplicate. No exact curve values extracted. — Figure 2A and caption; Results: Short-term proliferation and colony formation assays
MTT signal in additional non-malignant mesothelial models: Figure 3A–B: mean ± SEM of two independent experiments, each in duplicate; vehicle controls. — Figure 3A–B and caption; Results: Short-term proliferation and colony formation assays