In worms, Mianserin hydrochloride attenuated age-associated transcriptional changes in the comparison between age-matched treated and untreated controls within a design that also included FUDR. In another worm study whose design also included FUDR, Mianserin hydrochloride fully induced the xenobiotic response relative to water-treated controls irrespective of the treatment start time examined. In adult male crickets given Mianserin before recall testing, preference for the unpunished control odor increased significantly after aversive conditioning and reached levels as high as those in saline-injected controls. In fly brains, the octopamine-evoked intracellular calcium response was significantly decreased with octopamine plus Mianserin compared with octopamine alone. [1,2,3]
Literature-reported observations and conditions; not Adooq-tested protocols or general treatment recommendations.
Mianserin and age-associated transcriptional changes in worms
Animal study · N2 worms [1]
Animals in cohort #1 were treated on day 1 with water (solvent) or 50 µM mianserin, and harvested on day 3, 5, and 10 of adulthood. Mianserin treatment attenuates age-associated gene expression changes preserving expression levels as seen in young adults. Initiating mianserin treatment at later ages reduces (d3) or abolishes (d5) its effect on transcriptional drift.
Conditions shared by the study groups
- Treatment course
- treated on day 1 with water (solvent) or 50 µM mianserin, and harvested on day 3, 5, and 10 of adulthood
- Vehicle
- water (solvent)
- Mianserin hydrochloride 50 µM
- Model: N2 worms
- Reported limits: Not stated in the sentences used: route.
Methods and source locations
Compound reported in the paper: Mianserin hydrochloride; Compound label in the paper: Mianserin
Literature evidence for the named compound in the stated model; no Adooq product-use or lot-performance claim.
Results > Aging causes a loss of co-expression patterns observed in young adults (S5, S28); Materials and methods > RNA-sequencing (RNA-seq) transcriptional studies and data analysis (S292, S294, S295); Figure 2—figure supplement 2. legend (S406)
Mianserin-induced changes in transcription were determined by RNA-seq.: Mianserin-induced changes in transcription were determined by RNA-seq.; comparator: water (solvent) — Results > Aging causes a loss of co-expression patterns observed in young adults (S28); Materials and methods > RNA-sequencing (RNA-seq) transcriptional studies and data analysis (S292, S295)
Mianserin hydrochloride 50 µM · Results > Aging causes a loss of co-expression patterns observed in young adults (S28); Materials and methods > RNA-sequencing (RNA-seq) transcriptional studies and data analysis (S295)
Mianserin and aversive olfactory memory recall in crickets
Animal study · Adult male crickets, G. bimaculatus, at 1 week after the imaginal molt [2]
The effects of OA receptor antagonists on the recall of aversive olfactory memory were studied. The groups injected with epinastine and mianserin exhibited significantly increased preferences for the control odor, the levels of preferences being as high as that of the saline-injected control group. Thus, OA receptor antagonists do not impair aversive memory recall.
Conditions shared by the study groups
- Route
- into the head hemolymph
- Treatment course
- One day later; at 30 min after injection
- Vehicle
- saline
- Mianserin 1 μM
- Model: Adult male crickets, G. bimaculatus, at 1 week after the imaginal molt
Methods and source locations
Compound reported in the paper: Mianserin
The paper names Mianserin without stating the salt or form; not verified as the hydrochloride form supplied as A10588. No dose conversion or lot validation.
Results > OA and DA receptor antagonists impair the recall of appetitive and aversive olfactory memory, respectively (S16, S17, S18, S19); Methods > Insects (S145, S146); Methods > Olfactory conditioning procedure (S148); Methods > Pharmacology (S192); Figure 1 legend (S206, S207, S208, S210)
unpunished control odor: unpunished control odor; comparator: before and after conditioning; between experimental and saline-injected control groups — Results > OA and DA receptor antagonists impair the recall of appetitive and aversive olfactory memory, respectively (S18, S19); Figure 1 legend (S206, S207, S208, S210)
Mianserin 1 μM · Results > OA and DA receptor antagonists impair the recall of appetitive and aversive olfactory memory, respectively (S17, S18, S19); Methods > Pharmacology (S192); Figure 1 legend (S206, S207, S210)
Mianserin and octopamine-evoked calcium responses in fly brains
Animal study · Up to 7 day old uas-GCaMP3.0;0104-GAL4 flies; each n = 11 flies [3]
Fly brains received bath-applied Mianserin, and intracellular calcium responses were measured. The octopamine-evoked response was significantly decreased with octopamine plus mianserin compared with octopamine alone.
Conditions shared by the study groups
- Route
- added to the bath
- Treatment course
- The spontaneous baseline GCaMP3.0 response was imaged for 30 s; After another 30 s
- Vehicle
- sugar-free HL3-like saline
- Mianserin 2.45 mM
- Model: Up to 7 day old uas-GCaMP3.0;0104-GAL4 flies; each n = 11 flies
Methods and source locations
Compound reported in the paper: Mianserin
The paper names Mianserin without stating the salt or form; not verified as the hydrochloride form supplied as A10588. No dose conversion or lot validation.
Methods > In vivo calcium imaging (S61, S62, S64, S65); Figure 4 legend (S94, S95, S97, S98)
intracellular Ca2+, measured using GCaMP3.0, in 0104-DA neurons: intracellular Ca2+, measured using GCaMP3.0, in 0104-DA neurons; comparator: 5 mM OA; OA-evoked response (red trace) — Figure 4 legend (S94, S95)
Mianserin 2.45 mM · Methods > In vivo calcium imaging (S64, S65); Figure 4 legend (S95)
Mianserin and xenobiotic gene expression in worms
Animal study · N2 worms [1]
N2 worms received Mianserin hydrochloride in a design that also included FUDR. Treatment fully induced the xenobiotic response regardless of the reported treatment start time, compared with water-treated control animals.
Conditions shared by the study groups
- Treatment course
- on day 1 or day 5
- Vehicle
- water (solvent)
- Mianserin hydrochloride 50 µM
- Model: N2 worms
- Reported limits: Not stated in the sentences used: route.
Methods and source locations
Compound reported in the paper: Mianserin hydrochloride; Compound label in the paper: Mianserin
Literature evidence for the named compound in the stated model; no Adooq product-use or lot-performance claim.
Results > Longevity mechanisms attenuate transcriptional drift-variance (S63); Materials and methods > RNA-sequencing (RNA-seq) transcriptional studies and data analysis (S294, S297); Figure 2. legend (S369); Figure 2—figure supplement 2. legend (S406)
Log-fold change of xenobiotic gene expression on day 10: Log-fold change of xenobiotic gene expression on day 10; comparator: control animals treated with water on day 1 — Results > Longevity mechanisms attenuate transcriptional drift-variance (S63); Figure 2. legend (S369)
Mianserin hydrochloride 50 µM · Results > Longevity mechanisms attenuate transcriptional drift-variance (S63); Materials and methods > RNA-sequencing (RNA-seq) transcriptional studies and data analysis (S297); Figure 2. legend (S369)
