Developmental variation in muscimol-evoked calcium responses
Cell-based study · Acute VPm thalamic slices from C57BL/6J mice, P11, P14, P15 and P17 [1]
Cal-520-positive, SR101-negative neurons showed a smaller calcium-responsive fraction at P15 than P14. P11 versus P14 and P15 versus P17 comparisons were nonsignificant. Figure 5 reports 162, 83, 102 and 42 cells, respectively; these are cell counts, not animal counts.
| Endpoint | Reported result | Reference |
|---|---|---|
| Fraction of neurons with increased calcium fluorescence after muscimol, compared across ages | Between-age comparison P14 vs P15: p < 0.001; P11 vs P14 and P15 vs P17: n.s. | [1] |
Literature-Reported Treatment Conditions
- Treatment concentration
- 20 μM
- Method
- Bath application after a stable fluorescence baseline; images acquired every 3 s.
- Vehicle
- ACSF was the imaging medium; stock solvent was not specified in the imaging methods.
- Reported limits
- Exposure duration, stock solvent and animal-level replication for the imaging comparison are not specified in the relevant methods/caption. No antagonist control is reported for Figure 5 calcium imaging.
Methods and source locations
Compound reported in the paper: Muscimol hydrobromide; Compound label in the paper: Sigma M1523
Evidence concerns the named salt from Sigma. It does not establish Adooq lot performance or a receptor potency value.
Figure 5A–D and caption; Results paragraph immediately preceding Figure 5; Materials and Methods: Brain Slice Ca2+ Imaging; Discussion, final paragraph
Fraction of neurons with increased calcium fluorescence after muscimol, compared across ages: Confocal calcium imaging; Figure 5C significance brackets; caption specifies Student’s t-test and SEM. — Figure 5B–D image and caption; Brain Slice Ca2+ Imaging