In A549 cells, only 40 µg/mL Oleanolic Acid caused a notable decline in cell viability (56%). In neonatal primary microglial cultures, Oleanolic Acid increased PPAR-γ levels compared with untreated cultures. In a separate A549 study using Oleanolic Acid at 20 µg/mL, LC3-II expression was absent at 2 and 6 h and evident in the cytoplasm at 24 and 48 h. [1,2]
Literature-reported observations and conditions; not Adooq-tested protocols or general treatment recommendations.
Oleanolic Acid and A549 Cell Viability
Cell-based study · A549 cells [1]
A549 cells were treated with Oleanolic Acid and assessed for viability relative to untreated cells. Only 40 µg/mL caused a notable decline in cell viability (56%).
Literature-Reported Treatment Conditions
- Treatment concentration
- 5, 10, 20, and 40 µg/mL
- Exposure duration
- 24 and 48 h of incubation
- Method
- stimulated
- Reported limits
- Not stated in the sentences used: vehicle.
Methods and source locations
Compound reported in the paper: Oleanolic Acid
Literature evidence for the named compound in the stated model; no Adooq product-use or lot-performance claim.
2. Results > 2.1. Ursolic and Oleanolic Acids Decrease the Viability of A549 Human Lung Cancer Cells (S4); 4. Materials and Methods > 4.3. Cell Viability Assay (S82, S83, S84, S86); Figure 1 legend (S130, S131)
viability percentage: viability percentage; comparator: untreated cells (UNS) — 2. Results > 2.1. Ursolic and Oleanolic Acids Decrease the Viability of A549 Human Lung Cancer Cells (S4); 4. Materials and Methods > 4.3. Cell Viability Assay (S86)
PPAR-γ levels in neonatal primary microglial cultures
Cell-based study · Neonatal primary microglial cultures (2.5 × 105 cells) [2]
Neonatal primary microglial cultures were incubated with Oleanolic Acid at 4.5 μg/mL and 45 μg/mL for 24 h. Immunoblot analysis showed increased PPAR-γ levels specifically in microglia compared with untreated cultures.
Literature-Reported Treatment Conditions
- Treatment concentration
- 4.5 μg/mL and 45 μg/mL
- Exposure duration
- for 24 h
- Method
- incubation with oleanolic acid (OA)
- Reported limits
- Not stated in the sentences used: vehicle.
Methods and source locations
Compound reported in the paper: Oleanolic Acid
Literature evidence for the named compound in the stated model; no Adooq product-use or lot-performance claim.
Methods > PPAR-γ protein levels and ex vivo phagocytosis assay (S102, S103, S104); Results > M. parviflora hydroalcoholic extract regulates the phagocytic capacity of microglial cells via the PPARγ-CD36 pathway (S275); Fig. 12 legend (S407, S411)
The PPAR-γ levels from the cell extracts were determined by immunoblot: The PPAR-γ levels from the cell extracts were determined by immunoblot; comparator: left untreated — Methods > PPAR-γ protein levels and ex vivo phagocytosis assay (S103, S104); Results > M. parviflora hydroalcoholic extract regulates the phagocytic capacity of microglial cells via the PPARγ-CD36 pathway (S275); Fig. 12 legend (S411)
Oleanolic Acid and LC3-II Expression in A549 Cells
Cell-based study · A549 cells [1]
A549 cells were treated with 20 µg/mL Oleanolic Acid and assessed by immunofluorescence for LC3-II. Expression was absent at 2 and 6 h after stimulation but evident in the cytoplasm at 24 and 48 h after treatment.
Literature-Reported Treatment Conditions
- Treatment concentration
- 20 µg/mL
- Exposure duration
- 2, 6, 24, and 48 h
- Method
- stimulated
- Reported limits
- Not stated in the sentences used: vehicle.
Methods and source locations
Compound reported in the paper: Oleanolic Acid
Literature evidence for the named compound in the stated model; no Adooq product-use or lot-performance claim.
2. Results > 2.2. Ursolic and Oleanolic Acids Induce Autophagy in A549 Human Lung Cancer Cells (S8, S9); Figure 2 legend (S133, S134)
immunofluorescence analysis for LC3-II: immunofluorescence analysis for LC3-II; comparator: A549 cells unstimulated (Uns) — 2. Results > 2.2. Ursolic and Oleanolic Acids Induce Autophagy in A549 Human Lung Cancer Cells (S8, S9)
