In β-cateninΔ(ex3)/+ MEFs, OSI-027 treatment at 2.5 μM for 48 h caused methuosis-like cell death characterized by catastrophic vacuolization, as observed by TEM. In DAOY cells treated with increasing concentrations of OSI-027 for 5 days, the cell viability assay reported a value of 1.703 μM. In human THP-1 monocytic cells, OSI-027 treatment for 24 h significantly reduced ribosome abundance. [1,2,3]
Literature-reported observations and conditions; not Adooq-tested protocols or general treatment recommendations.
Methuosis-like cell death in mutant β-catenin MEFs
Cell-based study · β-cateninΔ(ex3)/+ MEFs [1]
β-cateninΔ(ex3)/+ MEFs were treated with OSI-027 at 2.5 μM for 48 h. TEM images showed methuosis-like cell death characterized by catastrophic vacuolization.
Literature-Reported Treatment Conditions
- Treatment concentration
- 2.5 μM
- Exposure duration
- 48 h
- Vehicle
- DMSO
- Reported limits
- Not stated in the sentences used: route.
Methods and source locations
Compound reported in the paper: OSI-027
Literature evidence for the named compound in the stated model; no Adooq product-use or lot-performance claim.
Results > Hyperactive β-catenin stimulation of macropinocytosis confers cells susceptible to OSI-027-triggered methuosis (S20); Figure 2 legend (S126)
TEM (Transmission electron microscope) images: TEM (Transmission electron microscope) images; comparator: DMSO — Results > Hyperactive β-catenin stimulation of macropinocytosis confers cells susceptible to OSI-027-triggered methuosis (S20); Figure 2 legend (S126)
Cell viability assessment in DAOY cells
Cell-based study · DAOY cells [2]
DAOY cells were treated with increasing concentrations of OSI-027 for 5 days. The cell viability assay reported a value of 1.703 μM.
Literature-Reported Treatment Conditions
- Treatment concentration
- increasing concentrations
- Exposure duration
- 5 days
- Vehicle
- DMSO
- Reported limits
- Not stated in the sentences used: route, control.
Methods and source locations
Compound reported in the paper: OSI-027
Literature evidence for the named compound in the stated model; no Adooq product-use or lot-performance claim.
Results > Alpelisib and OSI-027 inhibit PI3K/mTOR signalling and exhibit antineoplastic effects in medulloblastoma cells (S10); Materials and Methods > Cell culture and reagents (S67); Materials and Methods > Cell viability and apoptosis assays (S79); Figure 1 legend (S108)
cell viability: cell viability — Results > Alpelisib and OSI-027 inhibit PI3K/mTOR signalling and exhibit antineoplastic effects in medulloblastoma cells (S10); Figure 1 legend (S108)
Reduced ribosome abundance in human monocytic cells
Cell-based study · human THP-1 monocytic cells [3]
Human THP-1 monocytic cells were treated with OSI-027 for 24 h. Quantitative statistics of ribosomes showed significantly reduced ribosome abundance.
Literature-Reported Treatment Conditions
- Exposure duration
- 24 h
- Method
- treated with OSI-027
- Reported limits
- Not stated in the sentences used: dose, vehicle, control.
Methods and source locations
Compound reported in the paper: OSI-027
Literature evidence for the named compound in the stated model; no Adooq product-use or lot-performance claim.
Results > The Intersection Analysis of LLPS and mTOR (S128); Materials and Methods > Ultrastructure of THP-1 Cells (S251); 8 legend (S341)
quantitative statistics of ribosomes: quantitative statistics of ribosomes — Results > The Intersection Analysis of LLPS and mTOR (S128); 8 legend (S341)
