Pretreatment with 1 μM PIK-294 did not alter apoptosis in control, uninfected PMNs, as assessed by Annexin V-FITC/PI staining and flow cytometry. In LVS-infected PMNs, PIK-294 did not alter the rate of apoptosis, with the compound effect isolated by comparison with PMNs left in medium alone. [1]
Literature-reported observations and conditions; not Adooq-tested protocols or general treatment recommendations.
Apoptosis in control uninfected PMNs
Cell-based study · control, uninfected PMNs [1]
Control, uninfected PMNs were pretreated with 1 μM PIK-294. Apoptosis assessed by Annexin V-FITC/PI staining and flow cytometry was unaltered.
Literature-Reported Treatment Conditions
- Treatment concentration
- 1 μM
- Exposure duration
- 60 min; 4, 10, 18, and 24 hours
- Method
- pretreated
- Vehicle
- dimethyl sulfoxide (DMSO)
Methods and source locations
Compound reported in the paper: PIK-294
Literature evidence for the named compound in the stated model; no Adooq product-use or lot-performance claim.
Materials and Methods > Assessment of Neutrophil Apoptosis (S21); Materials and Methods > Inhibition of Signaling Pathways (S28); Results > PI3K Activity Is Required for Survival of Both Infected and Uninfected Neutrophils (S78); Results > PI3Kα Plays a Specific Role in Extended Survival of F. tularensis-Infected Neutrophils (S89, S97, S98); Figure 5 legend (S169, S170); Table 1 (T1.h1, T1.r10)
Apoptosis was quantified at 4, 10, 18, and 24 hours using Annexin V-FITC/PI staining and flow cytometry.: Apoptosis was quantified at 4, 10, 18, and 24 hours using Annexin V-FITC/PI staining and flow cytometry.; comparator: medium alone — Results > PI3Kα Plays a Specific Role in Extended Survival of F. tularensis-Infected Neutrophils (S97, S98); Figure 5 legend (S170)
Apoptosis in LVS-infected PMNs
Cell-based study · LVS-infected PMNs [1]
PMNs were treated with PIK-294 prior to further incubation in the presence and absence of LVS. Neither of these drugs altered the rate of apoptosis of control or LVS-infected PMNs.
Literature-Reported Treatment Conditions
- Treatment concentration
- 1 μM
- Exposure duration
- 60 min; 4, 10, 18, and 24 hours
- Method
- pretreated
- Vehicle
- dimethyl sulfoxide (DMSO)
- Co-treatment
- Other interventions in this design: F. tularensis LVS 20 bacteria/cell. Compound effect isolated by the comparison: PMNs were left in medium alone.
Methods and source locations
Compound reported in the paper: PIK-294
Literature evidence for the named compound in the stated model; no Adooq product-use or lot-performance claim.
Materials and Methods > Infection and Culture of Neutrophils (S12); Materials and Methods > Assessment of Neutrophil Apoptosis (S21); Materials and Methods > Inhibition of Signaling Pathways (S28, S29); Results > PI3Kα Plays a Specific Role in Extended Survival of F. tularensis-Infected Neutrophils (S89, S97, S98); Figure 5 legend (S169, S170); Table 1 (T1.h1, T1.r10)
Apoptosis was quantified at 4, 10, 18, and 24 hours using Annexin V-FITC/PI staining and flow cytometry.: Apoptosis was quantified at 4, 10, 18, and 24 hours using Annexin V-FITC/PI staining and flow cytometry.; comparator: medium alone — Results > PI3Kα Plays a Specific Role in Extended Survival of F. tularensis-Infected Neutrophils (S97, S98); Figure 5 legend (S170)
