Fig. 1 · Structure

Cat. No.: A10992
CAS No.: 1214265-57-2
Formula: C₂₄H₂₅ClN₆OS
Mol. Weight: 481

Documents & Downloads

WZ8040

Name: WZ8040
Brand: Adooq Bioscience
SKU: A10992
Availability: InStock

Cat. No. A10992 CAS 1214265-57-2

EGFR Inhibitor

WZ8040 is a novel EGFR inhibitors that suppress the growth of EGFR-T790M-containing cell lines and inhibit EGFR phosphorylation.

Free Aliquoting for Your Order

Tell us your preferred amount per vial. We’ll confirm the options.

Biological Activity

Target & Activity

Target	Activity	Value
EGFR (T790M)

Cellular Effect

Cell Line	Activity	Value	Description	Reference
BaF3	IC50	2 nM	Antiproliferative activity against gefitinib sensitive mouse BA/F3 cells expressing EGFR exon 19 del E746_A750 activating mutant after 72 hrs by MTS assay	[PMID: 26882288]
BaF3	IC50	32 nM	Antiproliferative activity against mouse BA/F3 cells expressing wild type EGFR after 72 hrs by MTS assay	[PMID: 26882288]
BaF3	IC50	9 nM	Antiproliferative activity against gefitinib resistant mouse BA/F3 cells expressing EGFR L858R/T790M double mutant after 72 hrs by MTS assay	[PMID: 26882288]
NCI-H1975	IC50	9 nM	Antiproliferative activity against gefitinib resistant human NCI-H1975 cells expressing EGFR L858R/T790M double mutant after 72 hrs by MTS assay	[PMID: 26882288]

In Vitro Studies

WZ8040 was evaluated in L. mexicana promastigotes cultivated in the presence of test compounds. The reported result was a dose–response curve for promastigote viability. [1]

Literature-reported observations and conditions; not Adooq-tested protocols or general treatment recommendations.

WZ8040 in L. mexicana promastigotes

Cell-based study · L. mexicana (strain MNYC/BZ/62/M379) promastigotes [1]

L. mexicana promastigotes were cultivated in the presence of WZ8040. A dose–response curve was reported for promastigote viability.

Measured endpoints — not treatment concentrations
Endpoint	Reported result	Reference
promastigote viability	Reported result Dose–response curve for WZ8040 in Leishmania promastigotes.	[1]

Literature-Reported Treatment Conditions

Exposure duration: cells were cultivated in the presence of test compounds for 24 h and incubated with 10% AlamarBlue™ Cell Viability Reagent (ThermoFisher) for 24 h
Method: cells were cultivated in the presence of test compounds
Vehicle: 1% DMSO
Reported limits: Not stated in the sentences used: dose.

Methods and source locations

Compound reported in the paper: WZ8040

Literature evidence for the named compound in the stated model; no Adooq product-use or lot-performance claim.

Methods > Leishmania Cell Culture & Genetic Manipulation (S31, S35, S36); Figure 6 legend (S100)

promastigote viability: promastigote viability; comparator: Untreated cells (1% DMSO) — Methods > Leishmania Cell Culture & Genetic Manipulation (S35); Figure 6 legend (S100)

Specifications

Manufacturer	Adooq Bioscience
Actions	Inhibitor
CAS Number	1214265-57-2
Applications	EGFR Inhibitor
Formula	C₂₄H₂₅ClN₆OS
Molecular Weight	481
SMILES	CN1CCN(CC1)C2=CC=C(C=C2)NC3=NC=C(C(=N3)SC4=CC=CC(=C4)NC(=O)C=C)Cl

Storage & Solubility

In Vitro Solubility

Reported Solubility

Solvent	Reported Solubility
DMSO	86 mg/mL (178.79 mM)
Water	Insoluble
Ethanol	3 mg/mL (6.23 mM)

Preparing Stock Solutions

Stock preparation is unavailable for this product molecular weight.

Calculated stock concentrations do not establish solvent solubility. Refer to the reported solubility data for experimental guidance.

Storage

Store powder at -20°C, keep desiccated.
In powder form, the chemical is stable for 36 months.
In solution, store at -20°C and use within 1 month to prevent loss of potency. Aliquot to avoid multiple freeze/thaw cycles.

Research References

[1] Catta-Preta CMC, Ramos PZ, Carnielli JBT, et al. Discovery and Characterization of Cell-Permeable Inhibitors of Leishmania mexicana CLK1 Using an In-Cell Target Engagement Assay. ACS Infect Dis (2025). PMID: 40994280 DOI: 10.1021/acsinfecdis.5c00610 Source material