Fig. 1 · Structure

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1-Oxyl-2,2,5,5-tetramethylpyrroline-3-carboxylate NHS ester

Active Acylating Agent

1-Oxyl-2,2,5,5-tetramethylpyrroline-3-carboxylate NHS ester is an active acylating agent primarily utilized for spin labeling studies. This reagent effectively modifies proteins and aminoacylated RNA species, facilitating investigations into conformational dynamics and interactions. Its ability to introduce a stable paramagnetic label makes it valuable for applications in electron paramagnetic resonance (EPR) spectroscopy and related biophysical methodologies.

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Specifications

Manufacturer Adooq Bioscience
CAS Number 37558-29-5
Applications Active Acylating Agent
Formula C13H17N2O5*
Molecular Weight 281.28
Synonyms OXYL-1-NHS
SMILES
O=C(N1OC(C2=CC(C)(N(C2(C)C)[O])C)=O)CCC1=O

Storage & Solubility

In Vitro Solubility

Reported Solubility

No reported solubility data are available for this product.

Preparing Stock Solutions

Stock preparation is unavailable for this product molecular weight.

Calculated stock concentrations do not establish solvent solubility. Refer to the reported solubility data for experimental guidance.

Storage

Store powder at -20°C, keep desiccated.
In powder form, the chemical is stable for 36 months.
In solution, store at -20°C and use within 1 month to prevent loss of potency. Aliquot to avoid multiple freeze/thaw cycles.

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