Fig. 1 · Structure
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3-Indoxyl phosphate sodium
Phosphatase
3-Indoxyl phosphate sodium is a chromogenic substrate specifically designed for alkaline phosphatase. Upon enzymatic action, it releases 3-indoxyl, which is subsequently oxidized to form insoluble indigo, allowing for colorimetric quantification at 660 nm. Additionally, in alkaline conditions, this compound can be reduced to produce water-soluble leucoindigo, measurable by colorimetric detection at 415 nm or voltammetric methods. This reagent is suitable for use in enzyme-linked immunosorbent assays (ELISAs) and other applications involving alkaline phosphatase activity assessment.
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Specifications
| Manufacturer | Adooq Bioscience |
|---|---|
| CAS Number | 3318-43-2 |
| Applications | Phosphatase |
| Formula | C8H6NNa2O4P |
| Molecular Weight | 257.09 |
| Synonyms | 3-IP sodium |
| SMILES | O=P(OC1=CNC2=C1C=CC=C2)(O[Na])O[Na] |
Storage & Solubility
In Vitro Solubility
Reported Solubility
No reported solubility data are available for this product.
Preparing Stock Solutions
| Concentration | |
|---|---|
Values use four significant digits. Prepare to the final solution volume; this is not the volume of solvent to add.
Stock preparation is unavailable for this product molecular weight.
Calculated stock concentrations do not establish solvent solubility. Refer to the reported solubility data for experimental guidance.
Storage
Store powder at -20°C, keep desiccated.
In powder form, the chemical is stable for 36 months.
In solution, store at -20°C and use within 1 month to prevent loss of potency. Aliquot to avoid multiple freeze/thaw cycles.