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3'-O-Acetyl-dATP

Sanger Sequencing

3'-O-Acetyl-dATP is an acetylated derivative of deoxyadenosine triphosphate (dATP) utilized as a chain-terminating nucleotide in Sanger sequencing. This reagent effectively inhibits DNA polymerase activity when incorporated into growing DNA strands, facilitating the generation of sequence-specific fragments. Its application is critical for accurate DNA sequencing and analysis in molecular biology research.

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Specifications

Manufacturer	Adooq Bioscience
CAS Number	28120-63-0
Applications	Sanger Sequencing
Formula	C₁₂H₁₈N₅O₁₃P₃
Molecular Weight	533.22
Synonyms	3'-O-Acetyl-2'-deoxyadenosine-5'-O-triphosphate
SMILES	NC1=NC=NC2=C1N=CN2[C@H]3C[C@H](OC(C)=O)[C@@H](COP(OP(OP(O)(O)=O)(O)=O)(O)=O)O3

Storage & Solubility

In Vitro Solubility

Reported Solubility

No reported solubility data are available for this product.

Preparing Stock Solutions

Stock preparation is unavailable for this product molecular weight.

Calculated stock concentrations do not establish solvent solubility. Refer to the reported solubility data for experimental guidance.

Storage

Store powder at -20°C, keep desiccated.
In powder form, the chemical is stable for 36 months.
In solution, store at -20°C and use within 1 month to prevent loss of potency. Aliquot to avoid multiple freeze/thaw cycles.