4-(3-Tosyl-2-(tosylmethyl)propanoyl)benzoic acid is a potent biochemical assay reagent that serves as a robust covalent linker, featuring a free carboxylic acid and two tosyl groups. The tosyl moieties can be displaced by thiol or amine nucleophiles through Michael addition, allowing for dual reactions. This functionality enables the "stapling" of two reduced cysteine residues within proteins, facilitating the reformation of disulfide bridges. Additionally, the carboxylic acid can participate in the formation of amides or esters, expanding its utility in biochemical research applications.
4-(3-Tosyl-2-(tosylmethyl)propanoyl)benzoic acid is a potent biochemical assay reagent that serves as a robust covalent linker, featuring a free carboxylic acid and two tosyl groups. The tosyl moieties can be displaced by thiol or amine nucleophiles through Michael addition, allowing for dual reactions. This functionality enables the "stapling" of two reduced cysteine residues within proteins, facilitating the reformation of disulfide bridges. Additionally, the carboxylic acid can participate in the formation of amides or esters, expanding its utility in biochemical research applications.
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