Fig. 1 · Structure
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4-Nitrophenyl palmitate
Lipase/Esterase Substrate
4-Nitrophenyl palmitate serves as a chromogenic substrate specifically for lipases and esterases. Upon enzymatic hydrolysis, it releases p-nitrophenol, enabling quantification of enzymatic activity through colorimetric detection at 410 nm. This reagent is instrumental in characterizing the activity of lipolytic enzymes from diverse sources, such as bacterial strains and mammalian tissues, including Burkholderia and porcine pancreatic lipase.
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Specifications
| Manufacturer | Adooq Bioscience |
|---|---|
| CAS Number | 1492-30-4 |
| Applications | Lipase/Esterase Substrate |
| Formula | C22H35NO4 |
| Molecular Weight | 377.52 |
| Synonyms | 4-Nitrophenyl hexadecanoate; p-Nitrophenyl Palmitate; pNpp |
| SMILES | CCCCCCCCCCCCCCCC(OC1=CC=C([N+]([O-])=O)C=C1)=O |
Storage & Solubility
In Vitro Solubility
Reported Solubility
No reported solubility data are available for this product.
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| Concentration | |
|---|---|
Values use four significant digits. Prepare to the final solution volume; this is not the volume of solvent to add.
Stock preparation is unavailable for this product molecular weight.
Calculated stock concentrations do not establish solvent solubility. Refer to the reported solubility data for experimental guidance.
Storage
Store powder at -20°C, keep desiccated.
In powder form, the chemical is stable for 36 months.
In solution, store at -20°C and use within 1 month to prevent loss of potency. Aliquot to avoid multiple freeze/thaw cycles.