Fig. 1 · Structure
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4-Nitrophenyl phenylphosphonate
5'-Nucleotide Phosphodiesterase Substrate
4-Nitrophenyl phenylphosphonate serves as a substrate for 5'-nucleotide phosphodiesterase, facilitating the study of enzyme kinetics and mechanisms. This compound is useful in research applications involving nucleic acid metabolism and phosphodiester bond hydrolysis. Its ability to release 4-nitrophenol upon cleavage enables colorimetric detection methods, making it a valuable tool in biochemical assays.
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Specifications
| Manufacturer | Adooq Bioscience |
|---|---|
| Actions | Substrate |
| CAS Number | 57072-35-2 |
| Applications | 5'-Nucleotide Phosphodiesterase Substrate |
| Formula | C12H10NO5P |
| Molecular Weight | 279.19 |
| SMILES | O=P(O)(C1=CC=CC=C1)OC2=CC=C(C=C2)[N+]([O-])=O |
Storage & Solubility
In Vitro Solubility
Reported Solubility
No reported solubility data are available for this product.
Preparing Stock Solutions
| Concentration | |
|---|---|
Values use four significant digits. Prepare to the final solution volume; this is not the volume of solvent to add.
Stock preparation is unavailable for this product molecular weight.
Calculated stock concentrations do not establish solvent solubility. Refer to the reported solubility data for experimental guidance.
Storage
Store powder at -20°C, keep desiccated.
In powder form, the chemical is stable for 36 months.
In solution, store at -20°C and use within 1 month to prevent loss of potency. Aliquot to avoid multiple freeze/thaw cycles.