Fig. 1 · Structure
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4-Nitrophenyl phosphate
Phosphotyrosine-Like Substrate
4-Nitrophenyl phosphate is a phosphotyrosine-like substrate commonly employed in activity assays for protein tyrosine phosphatases. This colorless compound hydrolyzes to yield a yellow 4-nitrophenolate ion, which can be quantitatively measured by absorbance at 405 nm. Its utility in biochemical assays makes it essential for research in signaling pathways and enzyme activity studies.
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Specifications
| Manufacturer | Adooq Bioscience |
|---|---|
| Actions | Substrate |
| CAS Number | 330-13-2 |
| Applications | Phosphotyrosine-Like Substrate |
| Formula | C6H6NO6P |
| Molecular Weight | 219.09 |
| Synonyms | p-Nitrophenyl phosphate |
| SMILES | O=P(O)(O)OC(C=C1)=CC=C1[N+]([O-])=O |
Storage & Solubility
In Vitro Solubility
Reported Solubility
No reported solubility data are available for this product.
Preparing Stock Solutions
| Concentration | |
|---|---|
Values use four significant digits. Prepare to the final solution volume; this is not the volume of solvent to add.
Stock preparation is unavailable for this product molecular weight.
Calculated stock concentrations do not establish solvent solubility. Refer to the reported solubility data for experimental guidance.
Storage
Store powder at -20°C, keep desiccated.
In powder form, the chemical is stable for 36 months.
In solution, store at -20°C and use within 1 month to prevent loss of potency. Aliquot to avoid multiple freeze/thaw cycles.