5-TAMRA Cadaverine is a biochemical assay reagent that enables the modification of carboxylic acid groups in the presence of coupling agents such as EDC or DCC, forming stable amide bonds. This compound can also reversibly connect to aldehydes and ketones to generate Schiff bases, which can subsequently be reduced to stable amine derivatives using sodium borohydride or sodium cyanoborohydride. While 5(6)-TAMRA cadaverine is widely utilized as an orange-fluorescent dye for protein staining, its application in labeling peptides and nucleotides is less common due to challenges with purification. Using single isomer TAMRA may enhance resolution during HPLC purification, which is advantageous for conjugation applications.
5-TAMRA Cadaverine is a biochemical assay reagent that enables the modification of carboxylic acid groups in the presence of coupling agents such as EDC or DCC, forming stable amide bonds. This compound can also reversibly connect to aldehydes and ketones to generate Schiff bases, which can subsequently be reduced to stable amine derivatives using sodium borohydride or sodium cyanoborohydride. While 5(6)-TAMRA cadaverine is widely utilized as an orange-fluorescent dye for protein staining, its application in labeling peptides and nucleotides is less common due to challenges with purification. Using single isomer TAMRA may enhance resolution during HPLC purification, which is advantageous for conjugation applications.
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