Fig. 1 · Structure
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AMPPD
Alkaline Phosphatase Substrate
AMPPD is a chemiluminescent substrate for alkaline phosphatase (APase). It undergoes hydrolysis by APase to produce an unstable dioxetane intermediate, which emits a chemiluminescent signal upon decomposition. This property enables the sensitive detection of biological molecules and is particularly valuable in quantitative enzyme-linked immunosorbent assays (ELISAs) for targets such as human tissue kininogen. AMPPD is suitable for applications in chemiluminescent detection of proteins and nucleic acids, facilitating research in various biological and biochemical studies.
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Specifications
| Manufacturer | Adooq Bioscience |
|---|---|
| Actions | Substrate |
| CAS Number | 122341-56-4 |
| Applications | Alkaline Phosphatase Substrate |
| Formula | C18H23O7P |
| Molecular Weight | 382.34 |
| Synonyms | Lumi-Phos Plus; Lumigen PPD; PPD |
| SMILES | COC1(C2=CC(OP(O)(O)=O)=CC=C2)C3(C(C4)CC5CC4CC3C5)OO1 |
Storage & Solubility
In Vitro Solubility
Reported Solubility
No reported solubility data are available for this product.
Preparing Stock Solutions
| Concentration | |
|---|---|
Values use four significant digits. Prepare to the final solution volume; this is not the volume of solvent to add.
Stock preparation is unavailable for this product molecular weight.
Calculated stock concentrations do not establish solvent solubility. Refer to the reported solubility data for experimental guidance.
Storage
Store powder at -20°C, keep desiccated.
In powder form, the chemical is stable for 36 months.
In solution, store at -20°C and use within 1 month to prevent loss of potency. Aliquot to avoid multiple freeze/thaw cycles.