Fig. 1 · Structure
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Biotin-16-dCTP
DNA Labeling Substrate
Biotin-16-dCTP is a biotinylated deoxycytidine triphosphate designed as a crucial substrate for DNA labeling. It can be enzymatically incorporated into the 3' end of DNA probes using terminal deoxynucleotidyl transferase, facilitating the biotinylation of probes and enhancing chemiluminescent detection of low-abundance targets such as specific tRNA isoacceptors in Northern blotting assays. Additionally, Biotin-16-dCTP can substitute conventional dCTP in the synthesis of single-stranded DNA via asymmetric polymerase chain reaction, enabling applications in bioconjugation and pull-down assays. Care should be taken to avoid repeated freeze-thaw cycles to maintain its efficacy for probe biotinylation.
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Specifications
| Manufacturer | Adooq Bioscience |
|---|---|
| Actions | Substrate |
| CAS Number | 2567941-52-8 |
| Applications | DNA Labeling Substrate |
| Formula | C32H51N8O17P3S |
| Molecular Weight | 944.78 |
| SMILES | O[C@H]1C[C@H](N2C(N=C(N)C(C#CCNC(CCCNC(CCCCCNC(CCCC[C@H]3[C@]4([H])[C@](NC(N4)=O)([H])CS3)=O)=O)=O)=C2)=O)O[C@@H]1COP(OP(OP(O)(O)=O)(O)=O)(O)=O |
Storage & Solubility
In Vitro Solubility
Reported Solubility
No reported solubility data are available for this product.
Preparing Stock Solutions
| Concentration | |
|---|---|
Values use four significant digits. Prepare to the final solution volume; this is not the volume of solvent to add.
Stock preparation is unavailable for this product molecular weight.
Calculated stock concentrations do not establish solvent solubility. Refer to the reported solubility data for experimental guidance.
Storage
Store powder at -20°C, keep desiccated.
In powder form, the chemical is stable for 36 months.
In solution, store at -20°C and use within 1 month to prevent loss of potency. Aliquot to avoid multiple freeze/thaw cycles.