In sensitized CBA mice, cortisone acetate given 24 h before oxazolone recall testing markedly reduced or abolished test-site cell migration and left very little or no epidermal vesication. These findings concern a qualitative skin histology endpoint in a contact-sensitivity model. [3]
Age comparison of cortisol generation after an oral cortisone acetate challenge
Animal study · Chow-fed male C57BL/6 wildtype mice in young (12-week) and old (24-month) cohorts defined at euthanasia; cortisone acetate challenge performed 6 weeks before euthanasia. [1]
Cortisol generated after oral cortisone acetate was used as an in-vivo index of hepatic 11β-HSD1 activity. Figure 1D showed no significant difference between young and old wildtype cohorts. The challenge measures cortisol generation, not glucocorticoid-receptor affinity or an anti-inflammatory efficacy endpoint.
Conditions shared by the study groups
- Route
- Oral gavage
- Frequency
- Single administration
- Treatment course
- 6 weeks before euthanasia; sample after 20 min
- Vehicle
- Water
- Young wildtype cohort
- Model: Chow-fed male C57BL/6 wildtype mice in young (12-week) and old (24-month) cohorts defined at euthanasia; cortisone acetate challenge performed 6 weeks before euthanasia.
- Reported limits: No vehicle-only arm shown in Figure 1D.
- Old wildtype cohort
- Model: Chow-fed male C57BL/6 wildtype mice in young (12-week) and old (24-month) cohorts defined at euthanasia; cortisone acetate challenge performed 6 weeks before euthanasia.
- Reported limits: No exact means or P value reported in the adopted text.
Methods and source locations
Compound reported in the paper: Cortisone acetate; Compound label in the paper: Cortisone acetate challenge
Evidence concerns the reported cortisone acetate material and model; no Adooq lot validation or equivalence to free cortisone or hydrocortisone acetate is established.
Design and methods: Mouse ageing protocol, Cortisone acetate challenge and cortisol generation after gavage, Statistical analysis; Results first glucocorticoid-metabolism paragraph; Figure 1D and caption
Cortisol generated after cortisone acetate challenge: GC/MS at 20 min after gavage; unpaired Student’s t-test. Figure 1 caption reports n = 8–9 animals per group; data presented as mean ± SEM. No exact means or P value transcribed from bars. — Design and methods: Mouse ageing protocol, Cortisone acetate challenge and cortisol generation after gavage, Statistical analysis; Results first glucocorticoid-metabolism paragraph; Figure 1D and caption
Young wildtype cohort · Design and methods: Mouse ageing protocol, Cortisone acetate challenge and cortisol generation after gavage, Statistical analysis; Results first glucocorticoid-metabolism paragraph; Figure 1D and caption
Old wildtype cohort · Design and methods: Mouse ageing protocol, Cortisone acetate challenge and cortisol generation after gavage, Statistical analysis; Results first glucocorticoid-metabolism paragraph; Figure 1D and caption
Reduced skin cellular response during oxazolone recall
Animal study · 3–4-month-old CBA mice sensitized to oxazolone and tested for recall on day 10; sex not specified in the shared animal methods. [3]
Compared with sensitized mice not receiving cortisone acetate, administration 24 h before recall testing abolished or markedly reduced cell migration to the test site and produced very little or no epidermal vesication. The outcome is qualitative histology; the study does not establish a molecular receptor mechanism.
- Group E: cortisone acetate before recall
- Model: 3–4-month-old CBA mice sensitized to oxazolone and tested for recall on day 10; sex not specified in the shared animal methods.
- Route: Subcutaneous, two sites
- Frequency: Single dose
- Treatment course: Day 9 after sensitization, 24 h before day-10 test
- Reported limits: Vehicle not specified; qualitative small-group histology.
- Group D: recall without cortisone acetate
- Model: 3–4-month-old CBA mice sensitized to oxazolone and tested for recall on day 10; sex not specified in the shared animal methods.
- Treatment course: Day-10 recall test; samples 4, 24 and 48 h later
- Reported limits: No vehicle injection described; no numeric scoring.
Methods and source locations
Compound reported in the paper: Cortisone acetate; Compound label in the paper: Adreson; Organon Laboratories
Evidence concerns the reported cortisone acetate material and model; no Adooq lot validation or equivalence to free cortisone or hydrocortisone acetate is established.
p. 674 shared methods; Table 2 p. 675; Results “Effect of cortisone acetate on recall (groups D and E)” pp. 679 and 682
Dermal cell infiltration and epidermal vesication after recall testing: Ear-skin histology using haematoxylin/eosin and methyl-green/pyronin. Table 2: one animal at 4 h, three at 24 h, one at 48 h after testing in each of groups D and E; these are animals, not replicates of a quantitative score. — p. 674 shared methods; Table 2 p. 675; Results “Effect of cortisone acetate on recall (groups D and E)” pp. 679 and 682
Group E: cortisone acetate before recall · p. 674 shared methods; Table 2 p. 675; Results “Effect of cortisone acetate on recall (groups D and E)” pp. 679 and 682
Group D: recall without cortisone acetate · p. 674 shared methods; Table 2 p. 675; Results “Effect of cortisone acetate on recall (groups D and E)” pp. 679 and 682
