SPR binding of Crocin II to human ANGPTL8
Biochemical assay · Immobilized human ANGPTL8 on a CM5 sensor chip; Biacore S200 SPR analysis [1]
Concentration-dependent sensorgrams were fitted using a 1:1 Langmuir kinetic binding model. Reported K_D was lower for Crocin II than for Crocin I. This establishes the reported assay fit, without establishing selectivity or a cellular degradation mechanism.
| Endpoint | Reported result | Reference |
|---|---|---|
| Fitted binding affinity for immobilized human ANGPTL8 | K_D 1.276 μmol·L−1 | [1] |
| Crocin I comparator binding affinity for immobilized human ANGPTL8 | K_D 16.8 μmol·L−1 | [1] |
Literature-Reported Treatment Conditions
- Treatment concentration
- 0.3125, 0.625, 1.25, 2.5 and 5 μmol·L−1 Crocin II; same series for Crocin I (Fig. 1h legends)
- Method
- Gradient analyte concentrations injected over immobilized human ANGPTL8; fitted with Biacore S200 Evaluation Software. PBSP running buffer is specified in the SPR methods.
- Reported limits
- Analyte solvent, explicit blank/reference-channel subtraction, fit uncertainty and SPR-specific replicate definition are not stated in the acquired SPR methods/caption. Overall Fig. 1 caption says mean ± SEM, n = 3, without identifying the replicate basis of the kinetic fit. No exposure duration is assigned from the plotted time axis.
Methods and source locations
Compound reported in the paper: Crocin II; Compound label in the paper: Crocin II (author designation; compared with Crocin I)
Applies to author-designated Crocin II in this SPR experiment; does not certify an Adooq lot, chemical purity or isomer composition.
Materials and methods, “Surface plasmon resonance (SPR) assay”; Fig. 1h and caption; Results, first subsection; Animals (Crocin II sourcing); Discussion (Crocin I/II structural distinction)
Fitted binding affinity for immobilized human ANGPTL8: SPR; multi-cycle kinetic analysis and 1:1 Langmuir model — Fig. 1h, lower sensorgram and printed K_D; Results, first subsection
Crocin I comparator binding affinity for immobilized human ANGPTL8: SPR; multi-cycle kinetic analysis and 1:1 Langmuir model — Fig. 1h, upper sensorgram and printed K_D; Results, first subsection