Desthiobiotin-PEG3-NHS ester serves as a biochemical assay reagent, acting as a single-ring, sulfur-free biotin analog that targets streptavidin. It exhibits comparable binding specificity to streptavidin, although with lower affinity, enabling the effective elution of desthiobiotinylated proteins from streptavidin affinity resin. This elution occurs under mild conditions through competitive displacement with free biotin, making it a valuable tool for various biochemical applications and protein purification techniques.
Desthiobiotin-PEG3-NHS ester serves as a biochemical assay reagent, acting as a single-ring, sulfur-free biotin analog that targets streptavidin. It exhibits comparable binding specificity to streptavidin, although with lower affinity, enabling the effective elution of desthiobiotinylated proteins from streptavidin affinity resin. This elution occurs under mild conditions through competitive displacement with free biotin, making it a valuable tool for various biochemical applications and protein purification techniques.
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