Fig. 1 · Structure
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L-Leucyl-4-nitroanilide hydrochloride
Biochemical Assay Reagent
L-Leucyl-4-nitroanilide hydrochloride serves as a substrate for biochemical assays assessing the activity of peptide bond-cleaving enzymes, particularly proteases. Upon enzymatic cleavage, it produces p-nitroaniline, which results in a yellow colorimetric change, facilitating the quantitative measurement of protease activity. This reagent is applicable for analyzing biological samples, including blood and tissue extracts, in various research contexts.
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Specifications
| Manufacturer | Adooq Bioscience |
|---|---|
| Actions | Substrate |
| CAS Number | 16010-98-3 |
| Applications | Biochemical Assay Reagent |
| Formula | C12H18ClN3O3 |
| Molecular Weight | 287.74 |
| SMILES | N[C@@H](CC(C)C)C(NC1=CC=C([N+]([O-])=O)C=C1)=O.Cl |
Storage & Solubility
In Vitro Solubility
Reported Solubility
No reported solubility data are available for this product.
Preparing Stock Solutions
| Concentration | |
|---|---|
Values use four significant digits. Prepare to the final solution volume; this is not the volume of solvent to add.
Stock preparation is unavailable for this product molecular weight.
Calculated stock concentrations do not establish solvent solubility. Refer to the reported solubility data for experimental guidance.
Storage
Store powder at -20°C, keep desiccated.
In powder form, the chemical is stable for 36 months.
In solution, store at -20°C and use within 1 month to prevent loss of potency. Aliquot to avoid multiple freeze/thaw cycles.