Fig. 1 · Structure

Cat. No.: A10577
CAS No.: 532-03-6
Formula: C₁₁H₁₅NO₅
Mol. Weight: 241.24

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Methocarbamol

Nav1.4 Channel Blocker

Methocarbamol is a central muscle relaxant that functions as a Nav1.4 channel blocker. It reversibly modulates the voltage dependence of Nav1.4 channel inactivation, providing insights into neuromuscular transmission. This compound is valuable for research into muscle spasms and pain syndromes, making it a pertinent tool for studies focused on neuromuscular disorders and related therapeutic interventions.

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In Vitro Studies

In SH-SY5Y cells incubated in complete medium, 30 μM Methocarbamol produced a time-dependent increase in cathepsin D levels in the cell medium. The drug-induced increase was abrogated by VAMP7 or ARL8B inhibition. [1]

Literature-reported observations and conditions; not Adooq-tested protocols or general treatment recommendations.

Methocarbamol increases extracellular cathepsin D in cultured cells

Cell-based study · SH-SY5Y cells [1]

SH-SY5Y cells incubated in complete medium with 30 μM Methocarbamol showed a time-dependent increase in cathepsin D levels in the cell medium. The drug-induced increase was abrogated by VAMP7 or ARL8B inhibition.

Measured endpoints — not treatment concentrations
Endpoint	Reported result	Reference
Levels of cathepsin D released into the cell medium	Reported result SH-SY5Y cells incubated in complete medium with 30 μM methocarbamol showed a time-dependent increase in cathepsin D levels in the cell medium; the drug-induced increase in extracellular cathepsin D was abrogated by VAMP7 or ARL8B inhibition.	[1]

Literature-Reported Treatment Conditions

Treatment concentration: 30 μM
Exposure duration: over 12 h
Method: incubated in complete medium
Vehicle: 0.1% DMSO

Methods and source locations

Compound reported in the paper: Methocarbamol

Literature evidence for the named compound in the stated model; no Adooq product-use or lot-performance claim.

Results > CA inhibition induces tau secretion by lysosomal exocytosis (S74, S85); Methods > Cell-based experiments > ELISA analysis of cathepsin D in cell medium (S284, S285, S287); Fig. 4 legend (S512)

Levels of cathepsin D released into the cell medium: Levels of cathepsin D released into the cell medium; comparator: control samples treated with DMSO at 4 h — Results > CA inhibition induces tau secretion by lysosomal exocytosis (S74, S85); Methods > Cell-based experiments > ELISA analysis of cathepsin D in cell medium (S284)

In Vivo Studies

In rho:eGFP–tau-WT heterozygous fish, Methocarbamol at 10 μM added to each well increased the zpr-3:zpr-1 ratio by 457.9% compared with water control. The design also included phenylthiourea (PTU), with the compound effect isolated by comparison with water control. [1]

Methocarbamol increases the zpr-3:zpr-1 ratio in fish

Animal study · rho:eGFP–tau-WT heterozygous fish [1]

Measured endpoints — not treatment concentrations
Endpoint	Reported result	Reference
zpr-3:zpr-1 ratio	zpr-3:zpr-1 ratio 10 μM: increase in the zpr-3:zpr-1 ratio of 457.9%	[1]

Conditions shared by the study groups

Route: added to each well
Vehicle: water

Study groups and reported outcomes
Group / dose	Comparator	Observed result	Reference
Methocarbamol 10 μM	water control	increase in the zpr-3:zpr-1 ratio of 457.9%	[1]

Methocarbamol 10 μM: Model: rho:eGFP–tau-WT heterozygous fish; Reported limits: Not stated in the sentences used: schedule.

Methods and source locations

Compound reported in the paper: Methocarbamol

Literature evidence for the named compound in the stated model; no Adooq product-use or lot-performance claim.

Results > Identification of methocarbamol from the compound screen (S15); Methods > Zebrafish experiments > Experimental crosses (S140); Methods > Zebrafish experiments > Primary screen > Treatments with compound library (S156); Extended Data Fig. 2 legend (S401, S402)

zpr-3:zpr-1 ratio: zpr-3:zpr-1 ratio; comparator: water control — Results > Identification of methocarbamol from the compound screen (S15); Methods > Zebrafish experiments > Primary screen > Treatments with compound library (S156); Extended Data Fig. 2 legend (S401)

Methocarbamol 10 μM · Results > Identification of methocarbamol from the compound screen (S15); Methods > Zebrafish experiments > Primary screen > Treatments with compound library (S156); Extended Data Fig. 2 legend (S401)

Specifications

Manufacturer	Adooq Bioscience
Actions	Blocker
CAS Number	532-03-6
Applications	Nav1.4 Channel Blocker
Formula	C₁₁H₁₅NO₅
Molecular Weight	241.24
SMILES	OC(COC1=CC=CC=C1OC)COC(N)=O

Storage & Solubility

In Vitro Solubility

Reported Solubility

No reported solubility data are available for this product.

Preparing Stock Solutions

Stock preparation is unavailable for this product molecular weight.

Calculated stock concentrations do not establish solvent solubility. Refer to the reported solubility data for experimental guidance.

Storage

Store powder at -20°C, keep desiccated.
In powder form, the chemical is stable for 36 months.
In solution, store at -20°C and use within 1 month to prevent loss of potency. Aliquot to avoid multiple freeze/thaw cycles.

Research References

[1] Lopez A, Siddiqi FH, Villeneuve J, et al. Carbonic anhydrase inhibition ameliorates tau toxicity via enhanced tau secretion. Nat Chem Biol (2025). PMID: 39482469 DOI: 10.1038/s41589-024-01762-7 Source material