In HEK293T cells treated at 5 µM for 6 h, differential gene expression analyses reported that Mitoxantrone had the most significant impact on gene expression. In MCF-7 cells exposed for 72 hr, Mitoxantrone increased lysosome number per cell 2.7-fold at sub-cytotoxic concentrations as low as 10 nM and 3.9-fold at 30 nM. Mitoxantrone exposure at 30 nM for 72 hr also increased mean lysosome size, measured as cross-sectional lysosomal area, 1.9-fold in MCF-7 cells. In HEK-293 cells transfected with empty vector, Mitoxantrone treatment for 96 h yielded a reported IC50 of 3.8±1.8 nM. [1,2,3]
Literature-reported observations and conditions; not Adooq-tested protocols or general treatment recommendations.
Mitoxantrone Effects on Gene Expression
Cell-based study · HEK293T cells [1]
HEK293T cells were treated with Mitoxantrone at 5 µM for 6 h, with untreated HEK293T cells as controls. Differential gene expression analyses reported that Mitoxantrone had the most significant impact on gene expression.
Literature-Reported Treatment Conditions
- Treatment concentration
- 5 µM
- Exposure duration
- for 6 h
- Reported limits
- Not stated in the sentences used: route, vehicle.
Methods and source locations
Compound reported in the paper: Mitoxantrone
Literature evidence for the named compound in the stated model; no Adooq product-use or lot-performance claim.
Results > A combination regimen optimally targeting the HS-assisted viral entry (S135, S136, S138); Materials and methods > qRT-PCR analysis of knockout or knockdown cells (S231, S232)
Differential gene expression analyses: Differential gene expression analyses; comparator: untreated HEK293T cells — Results > A combination regimen optimally targeting the HS-assisted viral entry (S136)
Mitoxantrone Exposure and Lysosome Number
Cell-based study · MCF-7 cells [2]
MCF-7 cells were exposed to Mitoxantrone for 72 hr. Lysosome number per cell increased 2.7-fold at sub-cytotoxic concentrations as low as 10 nM and 3.9-fold at 30 nM.
Literature-Reported Treatment Conditions
- Treatment concentration
- 1–100 nM
- Exposure duration
- 72 hr
- Method
- exposed
- Reported limits
- Not stated in the sentences used: vehicle, control.
Methods and source locations
Compound reported in the paper: Mitoxantrone
Literature evidence for the named compound in the stated model; no Adooq product-use or lot-performance claim.
RESULTS > Exposure of MCF-7 cells to hydrophobic weak base drugs induces an increase in lysosome number (S24, S25); Figure 3 legend (S97, S98, S99)
Lysosome number: Lysosome number — RESULTS > Exposure of MCF-7 cells to hydrophobic weak base drugs induces an increase in lysosome number (S24, S25); Figure 3 legend (S99)
Mitoxantrone Response in Empty Vector Transfected Cells
Cell-based study · HEK-293 cells transfected with empty vector [3]
HEK-293 cells transfected with empty vector received various concentrations of Mitoxantrone for 96 h. The reported IC50 was 3.8±1.8 nM.
Literature-Reported Treatment Conditions
- Treatment concentration
- various concentrations
- Exposure duration
- 96 h at 37°C in 5% CO2
- Method
- added to the cells
- Reported limits
- Not stated in the sentences used: vehicle, control.
Methods and source locations
Compound reported in the paper: Mitoxantrone
Literature evidence for the named compound in the stated model; no Adooq product-use or lot-performance claim.
MATERIALS AND METHODS > Cytotoxicity assays (S29, S30); Figure 3 legend (S123); Table 2 (T2.h1, T2.h2, T2.r1)
IC50 (nM): IC50 (nM) — Table 2 (T2.h1, T2.h2, T2.r1)
Mitoxantrone Exposure and Mean Lysosome Size
Cell-based study · MCF-7 cells [2]
MCF-7 cells were exposed to Mitoxantrone at 30 nM for 72 hr. Mean lysosome size, determined as cross-sectional lysosomal area, increased 1.9-fold.
Literature-Reported Treatment Conditions
- Treatment concentration
- 30 nM
- Exposure duration
- 72 hr
- Method
- exposed
- Reported limits
- Not stated in the sentences used: vehicle, control.
Methods and source locations
Compound reported in the paper: Mitoxantrone
Literature evidence for the named compound in the stated model; no Adooq product-use or lot-performance claim.
RESULTS > Exposure of MCF-7 cells to hydrophobic weak base drugs induces an increase in lysosome number (S22, S25)
mean size of lysosomes (determined as the cross-sectional lysosomal area): mean size of lysosomes (determined as the cross-sectional lysosomal area) — RESULTS > Exposure of MCF-7 cells to hydrophobic weak base drugs induces an increase in lysosome number (S25)
