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Laboratory reagents here cover biotin labeling, maleimide linkers and fluorescent indicators. Sulfo-NHS-Biotin and sulfosuccinimidyl-6-(biotinamido)hexanoate sodium are N-hydroxysuccinimide (NHS) esters that attach biotin to primary amines. Their sulfonate group makes them water-soluble, which suits labeling of cell surface proteins. Biotin Hydrazide reacts with aldehydes, for example on glycoproteins after periodate oxidation. Biotinyl Cystamine carries a disulfide bond that can be cleaved by reduction. 6-Maleimidocaproic acid provides a thiol-reactive maleimide on a six-carbon spacer for linker synthesis. Fluo-3 is a calcium indicator whose fluorescence rises on binding calcium ions, and PF6-AM is a cell-permeant probe for hydrogen peroxide. Single compounds such as monobenzone and hexanoyl glycine complete the set.
