PPM1A Human

Catalog No.: AP4491
Protein Phosphatase 1A Alpha Isoform Human Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 418 amino acids and having a molecular mass of 46.6KDa (containing a 36 aa His tag, T7 gene 10 leader, XpressTM Epitope). The protein coding region of PPM1A (amino acids 1-382) was cloned into an E. coli expression vector (BamHI/Hind3 site). PP2Ca was overexpressed in E. coli as a soluble His-tag fusion protein, and it was purified by conventional column chromatographic techniques.
Grouped product items
Size Price Stock Qty
10µg
$110.00
In stock
50µg
$290.00
In stock
1mg
$3,960.00
In stock
Bulk Size
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Protein Information
Catalog Num AP4491
Physical appearance Sterile filtered colorless solution.
Formulation PPM1A protein solution (1mg/ml) contains 10mM Tris-HCl, pH7.5, 50mM NaCl, 2mM DTT, 1mM MnCl2 and 20% glycerol.
Amino acid sequence MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWILMGAF LDKPKMEKHN AQGQGNGLRY GLSSMQGWRV EMEDAHTAVI GLPSGLESWS FFAVYDGHAG SQVAKYCCEH LLDHITNNQD FKGSAGAPSV ENVKNGIRTG FLEIDEHMRV MSEKKHGADR SGSTAVGVLI SPQHTYFINC GDSRGLLCRN RKVHFFTQDH KPSNPLEKER IQNAGGSVMI QRVNGSLAVS RALGDFDYKC VHGKGPTEQL VSPEPEVHDI ERSEEDDQFI ILACDGIWDV MGNEELCDFV RSRLEVTDDL EKVCNEVVDT CLYKGSRDNM SVILICFPNA PKVSPEAVKK EAELDKYLEC RVEEIIKKQG EGVPDLVHVM RTLASENIPS LPPGGELASK RNVIEAVYNR LNPYKNDDTD STSTDDMW.
Uniprot ACC# P35813
Purity Greater than 95.0% as determined by SDS-PAGE.
Stability Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid multiple freeze-thaw cycles.
Source Escherichia Coli.
Synonyms Protein phosphatase 1A, EC 3.1.3.16, Protein phosphatase 2C isoform alpha, PP2C-alpha, IA, PPM1A, PP2CA, MGC9201.
Transportation method Shipped with Ice Packs