CDK2 dependence of the cell-cycle response in mouse fibroblasts
Cell-based study · Immortalized wild-type and CDK2-null mouse embryonic fibroblasts [1]
R-roscovitine produced S-phase accumulation in wild-type MEFs; this response was absent in CDK2-null MEFs. The experiment supports CDK2 dependence of this cell-cycle phenotype.
| Endpoint | Reported result | Reference |
|---|---|---|
| S-phase accumulation after R-roscovitine exposure | Qualitative cell-cycle distribution S-phase accumulation in wild-type MEFs; prevented by CDK2 loss; representative of two experiments | [1] |
Literature-Reported Treatment Conditions
- Treatment concentration
- 10, 25 and 50 μM; untreated comparator
- Exposure duration
- 48 h
- Method
- Cell exposure followed by DNA-content flow cytometry
- Vehicle
- Untreated control shown; DMSO was used for compound stocks. A separately labeled matched-vehicle FACS arm is not specified in the retrieved protocol/caption.
- Reported limits
- No numerical phase percentages are extracted from the bars. Figure 11B is representative of two experiments; no inferential statistics for phase distributions are given in its caption.
Methods and source locations
Compound reported in the paper: R-roscovitine (seliciclib; CYC202); Compound label in the paper: R-roscovitine
Results apply to the reported R-roscovitine material and model; no supplier-lot or formulation equivalence is established.
Figure 11B and caption (p. 31218); Cell Cycle Analysis (p. 31211); CDK2-null MEF Results (pp. 31214–31215); Figure 10B (p. 31217)
S-phase accumulation after R-roscovitine exposure: DNA-content FACScan analysis following methanol fixation, RNase and propidium iodide staining — Figure 11B and caption (p. 31218); Cell Cycle Analysis (p. 31211); CDK2-null MEF Results (pp. 31214–31215); Figure 10B (p. 31217)